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Genetic Construct for Fluorescent Protein Expression in Bacterial Systems

This genetic construct features various fluorescent proteins including GFP, YFP, and RFP linked to different regulatory elements such as RBS (Ribosome Binding Sites) and promoters like PLac and PBAD. The design includes multiple degradation tags to control protein stability and expression levels. Restriction enzyme sites are also incorporated for convenient cloning, including EcoRI, SpeI, XbaI, and PstI. This versatile construct allows for controlled expression of fluorescent proteins in bacterial systems, facilitating studies in protein localization, dynamics, and interactions.

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Genetic Construct for Fluorescent Protein Expression in Bacterial Systems

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  1. 2:11I RBS Term Term PXyl GFP 2:11G 5:2M CFPdeg RBS Term PLac 3:15O Sub-PartA2 Sub-PartA1 YFPdeg RBS Term Term PHyc 5:6E 5:2M 1:15J 3:4F 5:23L RFP? RBS PBAD Addgene

  2. E S X P E S X P E S X P Phyc RBS E S X P E: EcoRI S: SpeI X: XbaI P: PstI

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