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Investigation of the BARREN STALK1 gene lineage in graminoid Poales

Investigation of the BARREN STALK1 gene lineage in graminoid Poales. Daniel Woods. rice. corn. Outline. Introduction into field of Evolutionary Developmental Genetics and Grasses 2)What’s BARREN STALK1 3) Hypothesis/aims 4) Preliminary Data. molecular evolution. morphological

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Investigation of the BARREN STALK1 gene lineage in graminoid Poales

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  1. Investigation of the BARREN STALK1 gene lineage in graminoid Poales Daniel Woods

  2. rice corn

  3. Outline Introduction into field of Evolutionary Developmental Genetics and Grasses 2)What’s BARREN STALK1 3) Hypothesis/aims 4) Preliminary Data

  4. molecular evolution morphological development functional analyses gene expression phylogenetics ? rice corn Graminoid Poales

  5. Chasmanthium (CEN) inflorescence development

  6. barren stalk1 (ba1) phenotype Wild-type tassel ba1-ref WT meristem ba1-mum1 Gallavotti, A., et al. (2004). Nature 432(7017): 630-5 Gallavotti, A., et al. (2004). Nature 432(7017): 630-5

  7. BA1/LAX expression via in situ hybridization Maize (ZmBA1) Rice (OsLAX) Komatsu, K., et al. (2003). Proc Natl Acad Sci USA100(20): 11765-70 Gallavotti, A., et al. (2004), Nature432(7017): 630-5

  8. Hypotheses: BA1 duplication event occurred at base of grass family BA1 is expressed in branching primordia throughout inflorescence development

  9. Specific aims to test hypothesis • Isolate BA1 bHLH genes from diverse array of grasses and near grass relatives 2) Generate phylogenies to pinpoint duplication event 3) Document inflorescence development using Scanning Electron Microscopy (SEM) 4) Gene Expression Analysis via in situ hybridization

  10. BEP PACCMAD

  11. Gene Isolation and Preparation Extractions Primer Design Polymerase Chain Reaction(Semi-nested) Gel Extraction Ligation Clone PCR screen Alkaline lysis Sequence(Sanger)

  12. Generate Phylogenies Maximum Parsimony Maximum Likelihood Bayesian

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