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qbol e.groenewald@cbs.knaw.nl

Identifying Quarantine Organisms with DNA Barcodes – QBOL and its Contribution to Q-bank presented by Ewald (J.Z.) Groenewald. www.qbol.org e.groenewald@cbs.knaw.nl. EPPO – European and Mediterranean Plant Protection Organization.

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qbol e.groenewald@cbs.knaw.nl

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  1. Identifying Quarantine Organisms with DNA Barcodes – QBOL and its Contributionto Q-bankpresented byEwald (J.Z.) Groenewald www.qbol.org e.groenewald@cbs.knaw.nl

  2. EPPO – European and Mediterranean Plant Protection Organization • Intergovernmental organization responsible for European cooperation in plant protection – www.eppo.org • EU Council Directive for legislation

  3. Problem definition • Species on Q lists not linked to specimens and associated metadata • Sometimes no sequence data or diagnostic protocols available • Morphology can be problematic (different larval stages, sterile fungal cultures, etc) • Taxonomy issues, e.g. different names for same species on different lists EPPO region

  4. QBOL – The project • Call: FP7-KBBE-2008-2B; Theme KBBE-2008-1-4-01: “Development of new diagnostic methods in support of Plant Health policy” • Small Collaborative Project in the Seventh Framework Programme of the EU • Project Proposal No.: 226482 • “Development Of A New Diagnostic Tool Using DNA Barcoding To Identify Quarantine Organisms In Support Of Plant Health” = QBOL • Run-time three years, starting 22/03/2009

  5. QBOL – 20 Global Partners

  6. QBOL – The 11 workpackages • WP1 – Coordination and Management • WP2-7 – Barcoding Fungi, Arthropods, Bacteria, Nematodes, Viruses & Phytoplasmas • WP8 – DNA Banks • WP9 – DNA barcode library / Databases / Informatics • WP10 – Validation / Evaluation • WP11 – Dissemination

  7. QBOL – Four main objectives • Obtain or produce relevant vouchered sequence data for individual pests or pest groups and position them in a correct taxonomic context – EU Council Directive and EPPO A1 and A2 lists • Develop generic diagnostic tools based on these barcode sequences • Enable the establishment of DNA banks and access to digital voucher specimens • Support better collaboration between EU and third-world country diagnostic laboratories

  8. QBOL – Overall progress • Midterm (21-22/09/2011) evaluation in Bologna, Italy, with EU representatives – Very positive feedback! • Number of sequences obtained (09/2011): • Fungi – 6,157 sequences (up to 8 loci) • Arthropods – 1,221 (up to 2 loci) • Bacteria – 1,749 (up to 20 loci) • Nematodes – 720 (up to 6 loci) • Viruses – 11 (whole genome) • Phytoplasms – 285 (up to 3 loci) • Data being added to Q-bank database – www.q-bank.eu (see poster D16)

  9. WP2 Fungi – Q barcode results Note: Species names according to the EPPO lists Mycosphaerella gibsonii http://photos.eppo.org/

  10. WP2 Fungi – Q barcode results Note: Species names according to the EPPO lists Ceratocystis fagacearum http://photos.eppo.org/

  11. QBOL – Conclusions • QBOL’s contribution to Q-bank database is essential for NPPO’s and other end-users • Links provided to specimens in physical collections (as far as possible) and nucleotide sequences extremely important • Identification with correct Q status irrespective of name used • Expand database with other Q-species not yet included (maybe international?)

  12. Special thank you to: • EU 7th Framework programme funding • All colleagues involved in QBOL • The international contributors and collaborators of QBOL Thank you for your attention! Visit www.qbol.org and www.q-bank.eu for more information. e.groenewald@cbs.knaw.nl Dothistroma septosporum Studies in Mycology 50:552

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