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Today: Lab notebooks due. Next week: M/T analyze gels Computer lab Turn in a one paragraph summary of your denaturation experiment (per PAIR) W/R CD: each PAIR will sign up for 25 minutes of CD time. Figure 6-11 Use of dialysis to separate small and large molecules. Page 139.

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slide1

Today: Lab notebooks due

Next week: M/T analyze gels

Computer lab

Turn in a one paragraph

summary of your denaturation

experiment (per PAIR)

W/R CD: each PAIR will sign up for

25 minutes of CD time

figure 6 32 isopycnic ultracentrifugation
Figure 6-32 Isopycnic ultracentrifugation.

Page 155

components of a mixture are separated on the basis of

differences in density

slide11

Theoretical considerations

http://www.bergen.org/AAST/Projects/Gel/technique2.htm

slide15

Ammonium persulfate: initiates the polymerization

NH4SO3-O-O-SO3NH4

S2O8-2 + 1e- SO4-2 + SO4-•

Free radical

TEMED: N,N-tetramethylenediamnine: catalyst

(CH3)2- N-CH2-CH2-N(CH3)2

SO4-• + n CH2=CH-CONH2 X-CH2-CH-CONH2

X-CH2-CH(CONH2)-CH2-CH(CONH2)-CH-CONH2

slide19

Structural features of most globular proteins:

1. Very compact:

e.g. Mb has room for only4 water molecules

in its interior.

2. Most polar/charged R groups are on the surface and

are hydrated.

3. Nearly all the hydrophobic R groups are on the interior.

4. Pro occurs at bends/loops/random structures

and in sheets

slide22

Simulation

http://www.rit.edu/~pac8612/electro/Electro_Sim.html

slide23
Figure 6-25 Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE.

log

slide24

Proteins in native gels exhibit a more

complex relationship between mobility

and MW.