Today: Lab notebooks due. Next week: M/T analyze gels Computer lab Turn in a one paragraph summary of your denaturation experiment (per PAIR) W/R CD: each PAIR will sign up for 25 minutes of CD time. Figure 6-11 Use of dialysis to separate small and large molecules. Page 139.
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Next week: M/T analyze gels
Turn in a one paragraph
summary of your denaturation
experiment (per PAIR)
W/R CD: each PAIR will sign up for
25 minutes of CD time
components of a mixture are separated on the basis of
differences in density
S2O8-2 + 1e- SO4-2 + SO4-•
TEMED: N,N-tetramethylenediamnine: catalyst
SO4-• + n CH2=CH-CONH2 X-CH2-CH-CONH2
1. Very compact:
e.g. Mb has room for only4 water molecules
in its interior.
2. Most polar/charged R groups are on the surface and
3. Nearly all the hydrophobic R groups are on the interior.
4. Pro occurs at bends/loops/random structures
and in sheets
complex relationship between mobility