microbial growth l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
MICROBIAL GROWTH. PowerPoint Presentation
Download Presentation
MICROBIAL GROWTH.

Loading in 2 Seconds...

play fullscreen
1 / 10

MICROBIAL GROWTH. - PowerPoint PPT Presentation


  • 312 Views
  • Uploaded on

MICROBIAL GROWTH. By Dr. Ijeoma Nkeiruka Otigbuo. PHYSICAL REQUIREMENTS. Temperature:Psychrophiles, mesophiles, thermophiles, and psychrotrophs. pH: Acidophiles, Extreme halophiles, and facultative halophiles. CHEMICAL REQUIREMENTS. Carbon. Nitrogen, Sulfur, and Phosphorus.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'MICROBIAL GROWTH.' - jana


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
microbial growth

MICROBIAL GROWTH.

By

Dr. Ijeoma Nkeiruka Otigbuo.

physical requirements
PHYSICAL REQUIREMENTS.
  • Temperature:Psychrophiles, mesophiles, thermophiles, and psychrotrophs.
  • pH: Acidophiles, Extreme halophiles, and facultative halophiles.
chemical requirements
CHEMICAL REQUIREMENTS.
  • Carbon.
  • Nitrogen, Sulfur, and Phosphorus.
  • Trace Elements.
  • Oxygen: Obligate aerobes, Facultative anaerobes, Obligate anaerobes, Aerotolerant anaerobes, and Microaerophiles.
  • Organic Growth Factors.
toxic forms of oxygen
TOXIC FORMS OF OXYGEN.
  • Singlet oxygen.
  • Superoxide free radicals.
  • The hydroxyl radical.
culture media
CULTURE MEDIA.
  • Chemically Defined Media.
  • Complex Media.
  • Anaerobic Growth Media and Methods.
  • Special Culture Techniques.
  • Selective and Differential Media.
  • Enrichment Culture.
obtaining pure cultures
OBTAINING PURE CULTURES.
  • Streak Plate Method.
preserving bacterial cultures
PRESERVING BACTERIAL CULTURES.
  • Deep Freezing.
  • Lyophilization.
  • Cryopreservation.
growth of bacterial cultures
GROWTH OF BACTERIAL CULTURES.
  • Bacterial Division.
  • Generation Time.
  • Phases of Growth: Lag, Log,Stationary, and Death.
direct measurement of microbial grotw
DIRECT MEASUREMENT OF MICROBIAL GROTW.
  • Pate Counts.
  • Serial Dilutions.
  • Pour Plates and Spread Plates.
  • Filtration.
  • Most Probable Number Method.
  • Direct Microscopic Count.
estimation of numbers by indirect methods
ESTIMATION OF NUMBERS BY INDIRECT METHODS.
  • Turbity.
  • Metabolic Activity.
  • Dry Weight.