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BioMEMS, Bio-Nanotechnology, and Agricultural Research. Michael R. Ladisch, Rashid Bashir, Arun Bhunia Laboratory of Renewable Resources Engineering, Departments of ABE, ECE, BME and Food Science Schools of Agriculture and Engineering Purdue University. Multi-disciplinary. Agriculture.

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BioMEMS, Bio-Nanotechnology, and Agricultural Research

Michael R. Ladisch, Rashid Bashir, Arun Bhunia

Laboratory of Renewable Resources Engineering,

Departments of ABE, ECE, BME and Food Science

Schools of Agriculture and Engineering

Purdue University


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Multi-disciplinary

Agriculture

Engineering

Biotechnology

Science

Medicine


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Nanoscience and Nanotechnology

Nanoscience:

Fabrication, study and modeling of devices and structures where at least one dimension is 200 nm or smaller.

Nanotechnology:

Enables devices that are compact, portable, energy efficient, integrate sensing, and carry out complex functions of a full-scale laboratory BioMEMS

Bio electromechanical systems


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Human Hair

75 µm

Plant and Animal Cells

Most Bacteria

Lysozyme

0.18µm

Transistor

Virus

Nanotechnology

Proteins

Carbon

Nano-tubes

Atoms

Nanotechnology: Size and Scale

100µm

10µm

1µm

Feature Size

100nm

10nm

1nm

0.1nm

Cooper, 2001


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Nanotechnology

Nano-Robots

Semiconductor

Technology

Molecular Electronics

“Wearable” Wireless

Internet Appliances

Vacuum Tube

Technology

Cell

Phones

The Internet

Performance

Computers

Television

Transistor

Radio

Radar

Radio

1900

1950

2000

2050

Time

Evolution of Technologies

Cooper, 2001


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Micro-Mechanical

Systems (MEMS)

Electronic Nanotechnology

Microfluidics

& Bio-Chips

Branches of Nanotechnology


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10 mm

Biochip:

Smaller than a Postage stamp

sealed channels and wells (volume of 1 to 10 nL/well)

electrodes for detection

connecting pads

fluidic I/O ports

Bashir et al.


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Role of Biotechnology

Provides

Bioreceptors

Biomarkers (targets)

Knowledge of mechanisms

Interfaces macro- and nano-worlds

Templates for design of small devices


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DNA mRNA Protein

Information

Component for Machine

Polymer of Amino Acids

Information

Assembly Point Disbands


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Opportunities for Research

Design and Assemble Technology Platform

Chips

Receptors

Bioseparations

Signal Transduction

Interface Hardware and Software

Component Assembly and Packaging


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Technology Issues

We must know:

Target molecule and its receptor

Threshhold for detection (sensitivity)

We must achieve:

Rapid sampling and conditioning

Specificity for target over biomolecules

High signal to noise


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The Vision

Detect Gene Expression Products

Applications

Food, Agriculture

Biosecurity

Animal Health

Medicine

Research, Industry, Hospital, Home


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Applications in Agriculture For Example-What is the cost of undetected pathogens?

Food Products

$13.1 billion in soda pop

7.5 billion in breakfast cereals

60.0 billion in fresh meats

Branded meats, precooked products,

micro-waveable red meats

Livestock, poultry

Sales figures from Kilman, Wall Street Journal, Feb 20, 2002


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BioMEMS - Devices and Systems

Combine micro and nano devices with biology

at the molecular level

Implanted Diagnostic and Therapeutic Devices

Organs

Implants

Pacemaker

Tissues

Drug Delivery

Drug Delivery

Polymers

Bottoms-Up

Biochips

Nano-bio

Organic/Biological

Inorganic


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Specific Objectives of National Research Program

1. Identify systems unique to agriculture

(cell membranes, starches, cellulose, oils)

2. Study biology of self-assembly processes

3. Apply to design, fabrication of BioMEMS

a. Tubes

b. Channels

c. Surfaces

Case studies must be agriculturally related


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Potential Outcomes and Impacts

  • Bio-inspired design of inorganic devices

  • Methods for mass-producing components for BioMEMS through agriculture

    a. A new fiber (cotton?) industry

    b. Self assembling oils

    c. Bio-based materials

    3. Sampling methods and detection of pathogens


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Research Budget Estimate

  • Bio-inspired assembly processes

    $ 1 million / year

  • Methods for assembling nano-components into micro-devices $1 million /year

  • Applications testing in the field

    $ 1 million / year


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