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Number of novel lncRNAs discovered

Why you need to sequence very deep to discover novel lncRNAs. Typical sequencing depth of most studies in the heart (50million PE-reads per sample). We sequenced to a depth of at least 300 million PE-reads per sample

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Number of novel lncRNAs discovered

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  1. Why you need to sequence very deep to discover novel lncRNAs........... Typical sequencing depth of most studies in the heart (50million PE-reads per sample) We sequenced to a depth of at least 300 million PE-reads per sample (Identified 100s of novel cardiac specific lncRNAs with unique characteristics) Ounzain S et al, EHJ, 2014 Number of novel lncRNAs discovered Ounzain S et al, JMCC, 2014 Missed novel lncRNAs at 50mil PE-reads Detected novel lncRNAs at 50mil PE-reads Average number of paired-end reads Sequencing depth per sample @ispiyou @CardiolncRNA

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