Lecture 7. Manipulation of foreign gene and secretion of foreign protein . Manipulation of gene expression . Isolation and Use of Different Promoters Targeted Alterations in Plant DNA Targeting foreign DNA into chloroplast genome. Isolation and Use of Different Promoters.
Manipulation of foreign gene and secretion of foreign protein
An example of a chimeric oligonucleotide used to change the nucleotide sequence of plant DNA. The 2’-O-methyl RNA residues are shown in lower case letters, the DNA residues are shown in uppercase letters
1.a fusion gene encoding the foreign protein and additional amino acids that direct the transport of the protein to the organelle inserted into the chromosomal DNA, and, after synthesis, the recombinant protein transported into the targeted organelle
2.the gene for the foreign protein inserted directly into chloroplast or mitochondrial DNA (Fig C)