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Design of Enzyme Reactor. Geoff Greer Joseph Gvildys Aaron McGregor April 2 nd , 2007. Enzyme Selection. Transpeptidation Trypsin or Achromobacter lyticus proteinase I Optimal enzyme conditions 4 o C and a pH of 7. Eli Lilly. Enzyme Immobilization. Benefits

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design of enzyme reactor

Design of Enzyme Reactor

Geoff Greer

Joseph Gvildys

Aaron McGregor

April 2nd, 2007

enzyme selection
Enzyme Selection
  • Transpeptidation
    • Trypsin or Achromobacter lyticus proteinase I
  • Optimal enzyme conditions
    • 4oC and a pH of 7

Eli Lilly

enzyme immobilization
Enzyme Immobilization
  • Benefits
    • Savings on enzyme requirements and downstream processing
  • Trypsin-Agarose
    • $211 for 100 units (~ 3mL)
reactor comparison
Reactor Comparison
  • Batch
    • High operating costs
    • Batch-to-batch variations
  • PFR
    • Difficult to control pH and Temperature
  • CSTR
    • Simple pH, Temperature control
    • Simple catalyst charging and replacement
reactor design
Reactor Design
  • Specify conversion, calculate [R]
  • From [R], calculate v
  • From v, calculate D
reactor design7
Reactor Design
  • From D, calculate V
  • From V, calculate tres
  • From Vmax, k2, calculate [Eo] and total amount of enzyme required
reactor sizing9
Reactor Sizing
  • Conversion of 99.25%
    • 120 L reactor required
  • Increase conversion by 0.5% to 99.75%
    • 360 L reactor required
enzyme required
Enzyme required
  • Required amount of enzyme is

1.83 μmol/L

    • 5.5 g pure trypsin
    • 2.6 kg immobilized trypsin
      • Based on difference in activity between immobilized and non-immobilized enzyme
      • ~ $18 000
reactor design considerations
Reactor Design Considerations
  • Temperature control
  • pH control
  • Level control
  • Sterilizable
  • Wire-mesh sieve
  • Sample port
costing of the reactor
Costing of the Reactor
  • Several different manufacturers
    • New Brunswick Scientific
    • Novaferm AB
    • Scigenics
    • New MBR
  • Quote from New Brunswick Scientific
    • BioFlo® Pro – 150 L (120 L working volume)
      • $140 000 (US)
    • Batch reactor
overall cost of unit
Overall Cost of Unit
  • For reactor and enzyme, estimated cost is at least $160 000
    • Additional considerations
      • Reactor modifications
      • De-naturation of enzyme
thank you
Thank You
  • Questions?