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Design of Enzyme Reactor

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  1. Design of Enzyme Reactor Geoff Greer Joseph Gvildys Aaron McGregor April 2nd, 2007

  2. Enzyme Selection • Transpeptidation • Trypsin or Achromobacter lyticus proteinase I • Optimal enzyme conditions • 4oC and a pH of 7 Eli Lilly

  3. Enzyme Immobilization • Benefits • Savings on enzyme requirements and downstream processing • Trypsin-Agarose • $211 for 100 units (~ 3mL)

  4. Reactor Comparison • Batch • High operating costs • Batch-to-batch variations • PFR • Difficult to control pH and Temperature • CSTR • Simple pH, Temperature control • Simple catalyst charging and replacement

  5. Design Parameters

  6. Reactor Design • Specify conversion, calculate [R] • From [R], calculate v • From v, calculate D

  7. Reactor Design • From D, calculate V • From V, calculate tres • From Vmax, k2, calculate [Eo] and total amount of enzyme required

  8. Reactor Sizing

  9. Reactor Sizing • Conversion of 99.25% • 120 L reactor required • Increase conversion by 0.5% to 99.75% • 360 L reactor required

  10. Enzyme required • Required amount of enzyme is 1.83 μmol/L • 5.5 g pure trypsin • 2.6 kg immobilized trypsin • Based on difference in activity between immobilized and non-immobilized enzyme • ~ $18 000

  11. Reactor Design Considerations • Temperature control • pH control • Level control • Sterilizable • Wire-mesh sieve • Sample port

  12. Simplified Reactor Schematic

  13. Costing of the Reactor • Several different manufacturers • New Brunswick Scientific • Novaferm AB • Scigenics • New MBR • Quote from New Brunswick Scientific • BioFlo® Pro – 150 L (120 L working volume) • $140 000 (US) • Batch reactor

  14. Final Reactor Schematic

  15. Overall Cost of Unit • For reactor and enzyme, estimated cost is at least $160 000 • Additional considerations • Reactor modifications • De-naturation of enzyme

  16. Thank You • Questions?