viruses n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
VIRUSES. PowerPoint Presentation
Download Presentation
VIRUSES.

Loading in 2 Seconds...

play fullscreen
1 / 50

VIRUSES. - PowerPoint PPT Presentation


  • 744 Views
  • Uploaded on

VIRUSES. Introduction. DISCOVERY OF VIRUSES. The discovery of viruses resulted from the search for the infectious agent causing tobacco mosaic plants and gives their leaves a mosaic coloration. ADOLF MEYER.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'VIRUSES.' - jacob


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
viruses

VIRUSES.

Introduction.

discovery of viruses
DISCOVERY OF VIRUSES.
  • The discovery of viruses resulted from the search for the infectious agent causing tobacco mosaic plants and gives their leaves a mosaic coloration.
adolf meyer
ADOLF MEYER.
  • A German scientist demonstrated that the disease was contagious and proposed that the infectious agent was an unusually small bacterium that could not be seen with a microscope.
  • He successfully transmitted the disease by spraying sap from infected plants onto the healthy ones.
d ivanowsky
D. IVANOWSKY.
  • 1890: A Russian scientist proposed that tobacco mosaic disease was caused by a bacterium that was either too small to be trapped by a filter or that produced a filterable toxin.
  • To remove bacteria, he filtered sap from infected leaves.
  • Filtered sap still transmitted disease to healthy plants.
martinus beijerinck
MARTINUS BEIJERINCK.
  • 1897: A Dutch microbiologist proposed that the disease was caused by a reproducing particle much smaller and simpler than a bacterium.
  • He ruled out the theory that a filterable toxin caused the disease by demonstrating that the infectious agent in filtered sap could reproduce.
slide7
Plants were sprayed with filtered sap from disease plants-----> sprayed plants developed tobacco mosaic disease----->sap from newly infected plants was used to infect others.
wendell m stanley
WENDELL. M. STANLEY.
  • 1935:An American Biologist, from the Rockefeller Institute, crystallized the infectious particle now known as Tobacco Mosaic Virus(TMV).
  • The purified virus precipitated in the form of crystals.
  • He was able to show that viruses can be better regarded as chemical matter, than as living organisms.
slide9
Crystals retained the ability to infect healthy tissue.
  • Subsequent determination of chemical nature of TMV: Protein in combo with nucleic, TMV were rods 300 nanometers long, TMV was RNA surrounded by protein coat.
general characteristics
GENERAL CHARACTERISTICS.
  • Obligatory Intracellular Parasites.
  • Protein coated fragments of DNA or RNA that have become detached from the genomes of cells. Because they cannot replicate on their own, they are not organisms.
  • Viruses are generally host-specific.
slide11
They reproduce only within a certain host.
  • Should be as many viruses as there are kinds of organisms.
virus host range
VIRUS-HOST RANGE.
  • The host range of a virus is the spectrum of host cells the virus can infect.
  • Some viruses have broad host ranges which may include several species(e.g. swine flu and rabies).
  • Some viruses have host ranges so narrow that they can:
  • infect only one species(e.g. phages of E.coli
slide13
Infect only a single tissue type of one species(e.g. human cold virus infects only cells of the URT; AIDS virus binds only to specific receptors on certain white blood cells.
viral size
VIRAL SIZE.
  • In the 1950’s, TMV and other viruses were finally observed with electron microscopes.
  • Smallest = 17 nanometers in diameter.
  • Largest = 1000 nanometers(1 micrometer) in greatest dimension.
  • Few are barely visible at light microscope level.
  • Most are visible only via EM.
viral structure
VIRAL STRUCTURE.
  • The virus or virion, is just nucleic acid enclosed by a protein coat, it’s a complete, fully developed infectious viral particle that is a vehicle of transmission from one host to another.
  • Viruses are classified by differences in the structures of these coats.
nucleic acid viral genomes
NUCLEIC ACID- VIRAL GENOMES.
  • Depending on the virus, viral genomes:
  • May be double-stranded DNA, single-stranded DNA, double-stranded RNA or single-stranded RNA.
  • Are organized as single nucleic acid molecules that are either linear or circular.
  • May have as few as four genes or as many as several hundred.
capsid and envelope
CAPSID AND ENVELOPE.
  • Capsid: Protein coat that encloses the viral genome.
  • It’s structure may be rod-shaped, polyhedral or complex.
  • Composed of many capsomeres: protein subunits made from only one or a few types of protein.
envelope
ENVELOPE.
  • Membrane that cloaks some viral capsid:
  • Helps viruses infect their host.
  • Derived from host cell membrane which is usually virus-modified and contains proteins and glycoproteins of viral origin.
general morphology
GENERAL MORPHOLOGY.
  • Helical viruses.
  • Polyhedral viruses.
  • Enveloped viruses.
  • Complex viruses.
helical viruses
HELICAL VIRUSES.
  • Resemble long rods.
  • Maybe rigid or flexible.
  • Viral Genome found inside a hollow cylindrical capsid.
  • E.G.: Ebola virus and Rabies virus.
polyhedral viruses
POLYHEDRAL VIRUSES.
  • Many-sided Capsid is in the shape of icosahedron( a polyhedral with 20 triangular faces).
  • E.G.: Adenovirus, and poliovirus.
enveloped viruses
ENVELOPED VIRUSES.
  • Roughly spherical.
  • Enveloped-helical or enveloped polyhedral viruses.
  • Enveloped helical = Influenzae virus.
  • Enveloped polyhedral = Herpes simplex virus.
complex viruses
COMPLEX VIRUSES.
  • Bacteriophage.
  • Capsid(head) is polyhedral, tail sheath is helical.
  • Tail fibers, plate and pin.
taxonomy of viruses
TAXONOMY OF VIRUSES.
  • Viruses are not organisms and are not classified in the kingdoms of life.
  • Regarded as self-replicating portions of the genomes of organisms.
  • In comparison to living things, vruses are acellular(not cells and do not consist of cells), do not metabolize energy, no photosynthesis, cell respiration or ferment.
slide26
Viruses are cell parasites as they infect organisms at all taxonomic levels.
  • Oldest classification based on symptoms.
  • ICTV group vruses into families, based on:
  • Nucleic acid type, strategy for replication, and morphology.
  • E.G.: Family= ends with viridae.
  • Genus= virus.
isolation cultivation and identification
ISOLATION, CULTIVATION, AND IDENTIFICATION.
  • Cannot replicate outside a living cell: poses problem with detection, ID, and cultivation.
  • Bacteriophages are easily grown on bacterial cultures.
  • This is the reason why so much of what is known of viral multiplication has come from from bacteriophage.
growing vruses in the lab
GROWING VRUSES IN THE LAB.
  • Plaque method: Bacteriophages.
  • Living animals: Animal viruses.
  • Embryonated eggs: Animal viruses.
  • Cell cultures: Animal viruses (CPE).
  • Primary cell lines, Diploid cell lines, and continuous cell lines.
viral multiplication
VIRAL MULTIPLICATION.
  • Viruses are nonliving particles that reproduce only inside specific host cells.
  • Exhibit many patterns of viral life cycle, but they generally include:
  • Coopting host ceel’s resources to:
  • Manufacture capsid protein,
  • Assemble newly produced viral nucleic acid and capsomeres.
slide30
Several mechanisms used to infect host cells with viral DNA.
  • For example, T-even phages use an elaborate tailpiece to inject DNA into the host cell.
slide31
Once the viral genome is inside its host cell, it commandeers the host’s resources and reprograms the cell to copy the viral genes and manufacture capsid protein.
slide32
Three possible patterns of viral genome replication:
  • 1. DNA---->DNA: If viral DNA is double-stranded, DNA replication resembles that of cellular DNA, and the virus uses DNA polymerase produced by the host.
slide33
2. RNA----->RNA: Since host cells lack the enzyme to copy RNA, most RNA viruses contain a gene that codes for RNA replicase
  • RNA replicase is an enzyme that uses viral RNA as a template to produce complementary RNA.
slide34
3. RNA----->DNA----->RNA: Some RNA viruses encode reverse transcriptase, an enzyme that transcribes DNA from an RNA template.
multiplication of bacteriophages
MULTIPLICATION OF BACTERIOPHAGES.
  • Studies on lambda phage of E. coli showed that double-stranded DNA viruses reproduce by two alternative mechanisms: The lytic cycle and the lysogenic cycle.
lytic and lysogenic cycles
Lytic cycle:

Results in the death or lysis of the host cell.

Bacteriophage takes over the machinery of the cell, so viral replication and release occur.

Lysogenic cycle:

Involves the incorporation of the viral genome into the host cell genome.

Phage becomes a prophage, integrated into the host genome.

LYTIC AND LYSOGENIC CYCLES.
slide37
Virulent bacteriophage reproduce by a lytic replication cycle= that lyse their host cells.

Later, the phage may reenter the lytic cycle and replicate itself.

Temperate viruses= integrate and remain latent.

the lytic cyce
THE LYTIC CYCE.
  • Attachment.
  • Penetration.
  • Biosynthesis.
  • Maturation.
  • Release.
the lysogenic cycle
THE LYSOGENIC CYCLE.
  • Lysogeny.
  • Prophage.
  • Phage conversion.
  • Specilized transduction.
multiplication of animal viruses
MULTIPLICATION OF ANIMAL VIRUSES.
  • Attachment.
  • Penetration.
  • Uncoating.
  • Biosynthesis of DNA viruses.
  • Biosynthesis of RNA viruses.
  • Maturation and Release.
viruses and cancer
VIRUSES AND CANCER.
  • Oncogenes.
  • Oncogenic viruses.
  • Contact Inhibition.
  • Tumor-specific transplantation antigen (TSTA ).
latent viral infections
LATENT VIRAL INFECTIONS.
  • Latent Infection:
  • Herpes simplex.
  • Chicken pox (varicella).
  • Shingles(zoster).
persistent viral infections
PERSISTENT VIRAL INFECTIONS.
  • Persistent viral infections: Encephalitis called subacute sclerosing panencephalitis(SSPE).
prions
PRIONS.
  • Proteinaceous infectious particle that lacks nucleic acid.
  • Nine animal diseases fall into this category.
  • All nine are neurological diseases called spongiform encephalopathies.
  • These diseases run in families, which indicates a possible genetic cause
spoke too soon
SPOKE TOO SOON.
  • However, The diseases cannot be purely inherited because mad cow disease arose from feeding scrapie-infected sheep meat to cattle, and the new (bovine) variant was transmitted to humans who ate undercooked beef from infected cattle.