Outline. CotinineMethodsChromatographyMass spectrometryIon suppressionValidationStability. Cotinine. Major metabolite of nicotine.Biomarker for nicotine exposure.Measured in urine, plasma, saliva, hair and semen.Half-life 19 hours.Nicotine metabolism only source of cotinine.. Smokers. Nicotine replacement and smoking cessation.Cotinine measurements are significantly higher in smokers compared to non-smokers.Quantitative method, monitor smoking habits..
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