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  1. Supplemental Figure 1. IgGs in brain parenchyma leaking from a disrupted blood-brain barrier. A: Using syndecan-1 immunohistochemistry, we observed numerous plasma cells in microvessel lumens but very few in the parenchyma of TLE tissues. B: To investigate IgG expression in various organs of naïve, mature rats, after blood sample collection, the rats were perfused with saline; brain and spleen were then dissected to perform Western blotting. Blood and spleen samples contained high concentrations of IgGs whereas no IgGs were found in brain extract samples. a1 = blood (150 µL), a2 = blood (70 µL), a3 = blood (10 µL), b1 = spleen, b2 = spleen, c1 = brain, c2 = brain. HC: heavy chain, LC: light chain.

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