In vitro infectivity of WNV ID NAT reactive plasmas that are positive for antibodies. Maria Rios, Ph.D. CBER/FDA Blood Product Advisory Committee July 22, 2005. Background. All reported cases of WNV transmission by blood transfusion have occurred during the acute, viremic phase
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In vitro infectivity of WNV ID NAT reactive plasmas that are positive for antibodies
Maria Rios, Ph.D.
Blood Product Advisory Committee
July 22, 2005
Single donor monocyte
Vero cells cultivated to 80% confluence
Cells cultivated in
DMEM + 10% FBS + M-CSF (1,000U/mL) + Gentamicin (10µg/mL)
Culture for 10 to14 days
Infection: medium removed, 0.5 mL of WNV +ve plasma + 4.5 mL of fresh medium added and incubated for 2 hr under gentle rocking
Culture medium containing 10 mL of fresh medium added and incubated at 37oC and 5% CO2
Culture observed daily for CPE
and/or TaqManin culture supernatants