Elettroforesi. (segue). SDS-PAGE. Pozzetti. Stacking gel. Running gel. Elettroforesi di proteine in condizioni native.
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(A) Analysis of binding of the b subunit cytoplasmic domain (bsol) to ECF1 and ECF1 (-). Mixtures of polypeptides were first analyzed by native agarose gel electrophoresis (A) through a 1% agarose gel.
Lanes from left to right:
I, ECF1 (-)
II, bsol ; III,
IV, bsol + ;
V, bsol + ( 1-134);
VI, bsol + ECF1 (-);
VII, + ECF1 (-);
VIII, bsol + + ECF1 (-);
IX, ECF1 (-) + ( 1-134);
X, bsol + ECF1 (-) + ( 1-134).
(B) Bands were excised from the agarose gel and separated on a 10-18% gradient of polyacrylamide in SDS.
Rodgers et al (1997) J. Biol. Chem. 272: 31058
Comparison of the sensitivity achieved with SYPRO, silver and Coomassie brilliant blue stains. Identical SDS-polyacrylamide gels were stained with A) SYPRO Orange protein gel stain, B) SYPRO Red protein gel stain; C) silver stain and D) Coomassie brilliant blue stain, according to standard protocols.
1 2 3 4 5 6
Lane 1: standard pei molecolari
Lane 2-6: campioni