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Peanut STS

Enzyme Structure & Mechanism. Peanut STS. Alfalfa C HS. Highly homologous overall structures. Reactions Catalyzed by CHS and STS. Different Cyclization in STS and CHS: Hydrolysis occurs ONLY during the Cyclization of STS. No thioesterase-like motif was found in STS.

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Peanut STS

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  1. Enzyme Structure & Mechanism Peanut STS Alfalfa CHS Highly homologous overall structures.

  2. Reactions Catalyzed by CHS and STS

  3. DifferentCyclization inSTS and CHS: Hydrolysis occurs ONLY during the Cyclization of STS No thioesterase-like motif was found in STS. How is the hydrolysis controlled in STS?

  4. (Nucleophilic) Water Molecule at the Active Sites of STSs Crystal structure of stilbene synthase from Arachis hypogaea. Shomura, Y, Torayama, I, Suh, D-Y, Kita, A, Sankawa, U and Miki, K (2005) Proteins60: 803

  5. Proposed “Aldol Switch” Hydrogen Bond Network at the STS Active Site STS Active Site 3.0Å 3.0Å 2.8Å (3.6Å) 2.8Å (3.5Å) 2.7Å 2.7Å (3.8Å) 2.7Å 2.7Å 2.7Å putative nucleophilic water / hydroxide ion An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases. Austin et al. Noel, JP (2004) Chem. Biol. 11:1179

  6. Reaction Sequence in the STS Cyclization: Hydrolysis Occurs First. ② ① CTAA

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