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a). b). raw image. Histogram of confidence measure. Histogram of confidence measure. Correction, segmentation. Histogram of confidence measure. Histogram of confidence measure. segmented image. Biological feature quantification. Robust regression. Histogram of final confidence measure.

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slide1

a)

b)

raw image

Histogram of confidence measure

Histogram of confidence measure

Correction, segmentation

Histogram of confidence measure

Histogram of confidence measure

segmented image

Biological feature quantification

Robust regression

Histogram of final confidence measure

c)

ellipse fits

Red/green normalization

clustering

Cell-shape, type heuristics

cell-type, stage assignment

Cell confidence

Typical object

Typical object

Typical object

Typical object

Typical object

Typical object

Typical object

Typical object

slide2

Expression intensity

a)

b)

Distance between

Distance to cell periphery

Distance to cell centre

Distance to pattern centre

bud

mother

CSC24

SLD3

PCL8

CDC6

ASH1

SIC1

daughter

lone cell

daughter

lone cell

daughter

lone cell

daughter

lone cell

mother

mother

mother

mother

bud

bud

bud

bud

PDR12

RSN1

TCB1

FTR1

MID2

TCB3

TCB2

YGR026W

MRH1

HXT6

SNQ2

MEP1

CDC6 bud

CDC6 bud

CDC6 bud

CDC6 mother

CDC6 mother

CDC6 mother

CDC6

SIC1 bud

SIC1 bud

SIC1 bud

SIC1 mother

SIC1 mother

SIC1 mother

SIC1

ASH1 bud

ASH1 bud

ASH1 bud

ASH1 mother

ASH1 mother

ASH1 mother

ASH1

FTR1 bud

FTR1 mother

FTR1 daughter

FTR1 lone cell

FTR1

HXT6 bud

HXT6 mother

HXT6 daughter

HXT6 lone cell

HXT6

slide3

c)

Distance to cell periphery

Distance to cell centre

bud

mother

bud

mother

STB1

WHI5

MCM4

MCM6

MCM7

MCM2

nucleus

nucleus

nucleus

nucleus

cytoplasm

cytoplasm

cytoplasm

cytoplasm

WHI5 bud

WHI5 bud

WHI5 bud

WHI5 bud

WHI5 mother

WHI5 mother

WHI5 mother

WHI5 mother

WHI5

MCM6 bud

MCM6 bud

MCM6 bud

MCM6 bud

MCM6 mother

MCM6 mother

MCM6 mother

MCM6 mother

MCM6

slide4

Expression intensity

Distance between

Distance to pattern cen.

Distance to periphery

Distance to cell centre

Distance to bud neck

Nucleus and cytoplasm (DNA binding and repair)

Vacuole

Nucleolus

Nucleus

Proteasome

Kinetochore

ER

Translation

Cytoplasm and nucleus (biosynthesis?)

Golgi

Mitochondrion

Vacuole

Vacuolar membrane

Cytoplasm

Cell periphery

Cytoplasm

Ambiguous, bud site

Nucleus and cytoplasm (DNA binding and repair)

ER

Mitochondrion

Bud neck

Golgi, peroxisome

ER