GOALS 1. Pouring Petri dishes for later experiments 2. Preparing broth medium, cotton-plug 3. Innoculating with live bacteria, 4. Streak-plate method 5. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method). Day 1. Microbiology Practical.
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Preparation of broth (Meat peptone broth)
Innoculating liquid samples onto medium
NA (nutrient agar)
Group 1-4 (mixture of Micrococcus luteus– yellow, small, Bacillus subtilis– cream white, large)
Group 5-8 (mixture of Serratiamarcescens red, small, E. coli, white small-medium size)
Goal:Learn the streak plate method
EMB agar (selective and differential medium)
All groups (mixture of E. coli and Proteus vulgaris)
Antimicrobial Agents (Kirby-Bauer Method)