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3D Quantification of Structures from Serial Histological Sections

Explore Neurolucida programs for tracing and analyzing branching neuron processes, mapping cells, measuring morphology, and morphometry. The Neurolucida system includes software, image overlay, microscope controls, and graphics output for accurate anatomical measurements and maps. Features high resolution, brightness intensity, and user-friendly controls. Contact R. Kraftsik for Volume Program on SGI and reconstruction of cortical neurons and dendritic segments from EM sections.

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3D Quantification of Structures from Serial Histological Sections

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  1. Quantification des structures 3D à partir de coupes histologiques sériées • R. Kraftsik.

  2. Programmes disponibles : • Neurolucida - PC • structures arborescentes, coupes sériée • Volume - SGI Indigo + G.T. • coupes sériées

  3. Neurolucida • Neuron Tracing: Trace and analyze branching neuron processes. • Brain Mapping: Map, count cells, and measure their locations and morphology • Morphometry: Make accurate anatomical maps and measurements of histologically distinct structures and regions

  4. Neurolucida system: -Neurolucida Software -Image Overlay Device (Optical and/or Video based) -Microscope Stage Control Device -Tracing Device -Graphics Output Device -PC Computer System.

  5. Neurolucida system, room 127 - color camera 640 X 480 - stepping stage + controller - Zeiss microscope, light and fluorescence Contact: R. Kraftsik

  6. Lucivid FEATURES: - Accurately overlays a computer graphics or text display onto the microscope image - High brightness intensity - Up to 1024x668 pixels resolution (PC and MAC) - Accessible controls for contrast and brightness - Does not interfere with conventional use of the microscope

  7. DRG - CR Sciatic nerve

  8. Volume Program, SGI Contact: R. Kraftsik

  9. Volume program, SGI. Contact R. Kraftsik Reconstruction of cortical neurons from EM sections

  10. Volume program, SGI. Contact R. Kraftsik Reconstruction of dendritic segments from EM sections

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