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ASCRS POSTER Category: Keratoconus/Ectasia

ASCRS POSTER Category: Keratoconus/Ectasia. Limbal Cell Damage Generated by Times of UVA Exposure in Animal Model With and Without Protection of Area Ramon Naranjo-Tackman,MD Cornea & Refractive Surgery Department Assn. to Prevent Blindness in Mexico

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ASCRS POSTER Category: Keratoconus/Ectasia

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  1. ASCRS POSTERCategory: Keratoconus/Ectasia Limbal Cell Damage Generated by Times of UVA Exposure in Animal Model With and Without Protection of Area Ramon Naranjo-Tackman,MD Cornea & Refractive Surgery Department Assn. to Prevent Blindness in Mexico The author has any financial interest in this poster

  2. Limbal Cell Damage • PURPOSE  • To explore in an animal model: The different degrees of tissue damage, generated by UV, With different times of exposure to UV radiation With and without protecting the limbal area Normal Guinea Pig Limbal area

  3. Limbal Cell Damage • MATERIAL & METHOD Guinea Pigs were chosen as the animal model Divided in four groups All ethics regulations for animal research, as well as internal hospital regulations were fulfilled The groups were divided according to the time of exposure and the use of protection over the limbus. UV was applied in Only one eye, using a CSO CXL device, previous application of riboflavine. Limbus was protected using an stained contact lens, perforated in the middle, exposing the central cornea. After 1 week, all animals were sacrificed and common Histologic stains and tunnel Studies (Looking for oxidative stress) were practiced.

  4. Limbal Cell Damage • RESULTS: 16 guinea pigs, were treated and sacrificed, Group1: (10 minutes of uvA exposition without limbal protection),Showed no caliciform cells, with oedema and inflammatory infiltration. Group2: (10 minutes with limbal protection). More caliciform cells Group3: (30 mins. Exposure to UV without limbal protection) Almost no caliciform cells, intense oedema & inflammatory cells- Group4: (30 mins, with protection).Slight diminishment of caliciform cells, limited edema and inflammatory cells.

  5. Limbal Cell Damage • RESULTS: Limbal region with infiltrates in 10mins. Infiltration without protection Tunnel control, No Tx

  6. Limbal Cell Damage 10 mins with protection 30 mins with protection

  7. Limbal Cell Damage 10 mins without protection 30 mins without protection

  8. Limbal Cell Damage • CONCLUSIONS: It is well known that cells are damaged by UV radiation, the present study shows damage to the limbal cells in the Guinea pigs model. The tunnel stain, showed different degrees of damages according to the time of exposure, and the protection or not of the limbus. CXL is gaining popularity, even when we don’t know the potential risks in the long term, specially for pluripotential cells like the limbal ones, so we should at least Protect them in every procedure, since we still haven’t tested the effect of time.

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