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COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE DE L ’ ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE. Authors:

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slide1

COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALSETUDE COMPARATIVE DE L’ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE

Authors:

Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU,

Dr. Gabriela MARCUS, student Adriana COJOCARU

University of MedicineandPharmacy Targu-Mures

ROMANIA

slide2
Aim
  • To assess the effect of homeopatic remedies in laboratory animals in placebo controled studies
  • Do homeopaticremedies act through placebo effect?
material and met h od
Material and method
  • Mice and rats from the bio-base (experimental station) of the University of Medicine and Pharmacy Targu-Mures
  • Various induced experimental pathology followed by treatment
    • homeopathic,
    • allopathic
    • combined, homeopathic and allopathic
experiment s
Experiments

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

The antitrauma effect of the remedyArnica in laboratory mice

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedyBelladonna

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

Homeopathic treatment of grade I burns in ratswith Apis and Belladonna

slide5
The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision
slide6
The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision
  • Previous studies made around the world with homeopathic Arnica –contradictory results
  • experimental studies with Staphysagria - missing
  • What are the benefits in surgery?
  • Our hypothesis – the need of administration of the remedy earlyafter surgery
material and method

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and method
  • 75 Wistar rats, weight 250-270 g
  • 5 groupsof 15 animals
material and method surgery technique

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and methodSurgery technique

Step 1 - tranquilization of the rats with ether

material and method surgery technique1

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and methodSurgery technique

Step 2 - preparing the surgery zone by depilation and disinfection

material and method surgery technique2

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and methodSurgery technique

Step 3 - performing a deep incision on the external side of the thigh, following the line of the femur until the appearance of the bone

material and method surgery technique3

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and methodSurgery technique

Step 4 - skin suture in 3 separate points with nonresorbable silk thread

material and method tr e a t ment

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and methodTreatment

Each rat was treated through a plastic probe, starting immediately after surgery and daily administration afterwards until the complete healing of the incision

material and method tr e atment

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and methodTreatment

The homeopathic remedies were dissolved in water, 1 pellet in 1 ml water

  • Group1- control group, sugar water as placebo,
  • Group2- Arnica CH 7,
  • Group3- Arnica CH 30,
  • Group 4 - Staphysagria CH 7,
  • Group 5 - Staphysagria CH 30.
material and method1

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Material and method

the length of the remaining incision was measured each day

the number of days necessary for the complete healingwas noted for each rat

results

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Results

The rats treated homeopathically, both remedies and both dilutions, showed a shorter healing time (15-16 days) than the placebo group (21-22 days), with a statistically significant difference (p<0,001, t-test)

the antitrauma effect of the remedy arnica in laboratory mice1
The antitrauma effect of the remedy Arnica in laboratory mice
  • 40 AKR2 white mice,
  • 2 groups of 20 mice: control, Arnica
  • Kocher clamp trauma at the level of the posterior limb
    • fractures
    • swelling
    • pain = protection of the affected limb, adynamia
  • Assesment of the variation of the dorsoplantar diameter
results1

The antitrauma effect of the remedy Arnica in laboratory mice

Results
  • Control group
    • Persistent swelling more than 180 minutes
    • adynamia
    • Protection of the limb
  • Arnica CH7 group
    • Moderate swelling, gone in less than 105 minutes (p < 0,001)
    • Good general state
    • Ability to use the affected limb, even fractured
    • normal activity
slide19
The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedyBelladonna
slide20
The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedyBelladonna
  • Pilocarpine – parasympathomimeticeffect – salivation in mice
  • Atropine(Atropa Belladonna)- parasympatholyticeffect, blocking of the muscarinic receptors, imposibility for pilocarpine to produce salivation
  • Belladonnain homeopatic dilution – does it compensatethe atropinic intoxication?
material and method2

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Material and method

80 de white mice AKR2 line divided in 4 groups:

  • Assessment of the salivation produced by pilocarpine (administration of pilocarpine).
  • Assessment of the muscarinic receptors blockade by atropine, given pilocarpine (administrationof atropine, then pilocarpine).
  • Assessment of supposed compensation of muscarinic blockade of atropine by homeopathic Belladonna CH7 (administrationof atropine, then Belladonna CH7, then pilocarpine).
  • Control group, assessment of physiologic salivation (administration of placebo).
material and method3

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Material and method

Step 1 – subcutaneous injection of Atropine 0,1%

material and method4

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Material and method

Step 2 - Belladonna CH7 given by gastric probe

material and method5

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Material and method

Step 3 – tranquilization with ethylic urethan intraperitoneally 1,5 g/kgc

material and method6

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Material and method

Step 4 - subcutaneous injection with 1% clorhidric pilocarpine

material and method7

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Material and method

The mice were put on filter paper

The area of salivary impregnation was measured each 10 minutes for 30 minutes

results2

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Results

The physiologic salivation was low, nearly immeasurable on the filter paper – group 4

results3

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Results

Pilocarpine produces an abundant salivation (known pharmacologic effect), assessed through the saliva spot area on the filter paper – group 1

results4

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Results

Atropine given before pilocarpine prevented the salivation, which wasvery low, nearly immeasurable on the filter paper (known pharmacologic effect) – group 2

results5

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Results

If homeopathic Belladonna CH 7 is given after atropine and before pilocarpine, the abundant salivation is present – group 3

results6

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Results

The variation of salivationin the first 30 minutesin the 4 groups

discussions

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Discussions
  • In mice the homeopathic remedy Belladonna in CH 7 dilution was able to give the atropinized organism the ability to be stimulated by pilocarpine.
  • It is possible, in theory, that the action of the homeopathic remedy Belladonna CH 7 could be one of the following:
    • it acted on the muscarinic receptors, determining them to release the atropine molecules and become sensible again to pilocarpine stimulation;
    • it acted on the atropine molecules, determining them to release the receptors on which they were bound;
    • it acted on cellular level, determining possible alternative ways for the de processing of the stimulus represented by pilocarpine (activation of new receptors, alternative biochemical or neuroendocrine ways)
slide33
The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice
slide34
The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice
  • Stricnina(Strychnos nux-vomica L) - adynamia, tonico-clonic convulsions, hypersensitivity to sensorial stimuli, deformation of visual and acoustic perceptions, tachycardia, tachypnea, agitation and finally death.
  • Diazepamul, benzodiazepină - tranquilizing, muscle-relaxant, anticonvulsant
  • Nux Vomica, Strychnos nux vomica seeds dilutions –used in cases of convulsions, aggravated by external stimuli, agitation, hypersensitivity to stimuli
material and method8

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Material and method

3 groups of 20 white mice - experimental poisoning - intraperitoneal injection of 1,5 mg/kg strychnine

  • Group 1 - diazepam5 mg/kg subcutaneous 1 hour before strychnine
  • Group 2 - Nux Vomica CH7orally by plastic probe immediatelly after strychnine
  • Group 3 - control (placebo), physiologic serum subcutaneous, sugar water by plastic probe
material and method9

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Material and method
  • Strychnine poisoning:
    • adynamia
    • tachycardia
    • tachypnea
    • agitation
    • tonico-clonic convulsions
    • death
material and method10

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Material and method

We assessed:

  • The time until convulsions
  • The number of animals which had convulsions
  • The time until death
  • The number of animals which died
results7

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Results

The time until convulsions

  • 180-390 sec Placebo group
  • 240-570 sec Diazepam group
  • 660-1080 sec homeopathic Nux Vomica group
results8

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Results
  • Convulsions present 100% in the Placebo and Diazepam group
  • 70% of mice had convulsions in the homeopathic Nux Vomica group
results9

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Results
  • Placebo groupmicedied in 240-610 sec
  • Diazepamtreated mice lived longer, 370-685 sec
  • Homeopathic Nux Vomica treatedmice showed the longest survival, 605-1150 sec
results10

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Results
  • All the mice (100%) in the Placeboand Diazepamgroup died
  • Homeopatically treated mice with Nux Vomicadied in 80%
slide42

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

slide43

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

  • Formaldehyde– mastocyt reaction - histamine
  • Apis - swelling, redness, pain, warmth
  • Hydrocortisone Hemisuccinate (HHS) - steroidal anti-inflammatory
  • Diclofenac - nonsteroidal anti-inflammatory
material and method11

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

Material and method
  • 70 white mice – local swellingin the posterior limb by subcutaneous formaldehyde 0.5%
  • Dorsoplantar diameter was measured before formaldehyde and then each 15 minutes
material and method12

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

Material and method

7 groups of 10 mice

  • Hydrocortisone Hemisuccinate
  • Diclofenac
  • Apis CH 7
  • Apis CH 15
  • Hydrocortisone Hemisuccinate and Apis CH 7
  • Hydrocortisone Hemisuccinate and Apis CH 15
  • Control group, placebo
results11

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

Results
  • Local signs of inflammation
    • redness
    • swelling
    • motor impairment
    • agitation
    • protection of the limb
results12

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

Results

Dorsoplantar diameter evolution in time(average values)

homeopathic treatment of grade i burns in rats with apis and belladonna material and met h od
Homeopathic treatment of grade I burns in ratswith Apis and BelladonnaMaterial and method
  • 3 groups of 10 rats
  • Tranquilization with ether
  • Immersion of posterior left limb in hot water at 55°C for 20 seconds
material and method13
Material and method

Grade I burns - edemaand erithema

material and method14
Material and method

Treatment by gastric probe:

  • Group 1 - placebo as sugar water
  • Group 2 - Apis CH9
  • Group 3 - Belladonna CH9
material and method15
Material and method

Assessment:

  • The number of healed rats each day
  • The average number of days necessary for the complete healing for each group
results13
Results
  • Apis CH9 group showed the fastest healing, followed by the Belladonna CH9 group
  • The Placebo group showed the longest healing time
results14
Results
  • Apisgroup healed in the days 1-2, with 80% rats in the first day
  • Belladonnagroup healed in the days 1-3
  • Placebogroup healed in the days 2-4
conclusions 1 4
Conclusions(1/4)
  • The homeopathic remedies Arnica and Staphysagria in the dilutions CH 7 and CH 30, given early after surgery, had a significant effect in the reduction of the healing time of the surgical incision in rats
  • The homeopathic remedy Arnica CH 7 fights the effects of mechanic trauma in mice if given immediately after trauma
  • In mice the homeopathic remedy Belladonna in CH 7 dilution was able to give the atropinized organism the capability to be stimulated by pilocarpine, even if the specific receptors were blocked
conclusions 2 4
Conclusions(2/4)
  • Homeopatic Nux Vomica CH7 was significantly more efficient than Diazepam in delaying and even preventing the convulsions in the experimental strychnine poisoning. The time until death was significantly prolonged, some of the mice treated homeopathically surviving the experiment
  • The anti-inflammatory effect of the remedy Apis in our experiments had a higher intensity than Hydrocortisone Hemisuccinate and Diclofenac, without being antidoted by them in combinations
  • Apis CH9 wad more efficient than Belladonna CH9 in the treatment of grade I burns in rats, both being more efficient than placebo
conclusions 3 4
Conclusions(3/4)

In all our experiments, the effect of homeopathic remedies was objectively assessed, reproducible and statistically significant

Homeopathy is different from placebo

Homeopathy acts quickly in acute

Homeopathy can be associated with allopathy

conclu sions 4 4
Conclusions(4/4)

Homeopathic research must be extended

C'est la guerre!