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MASS SPECTROMETRY INTRODUCTION. Ilyana Mushaeva and Amber Moscato Department of Electrical and Computer Engineering Temple University. Spectrometry vs. Spectroscopy.

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MASS SPECTROMETRY

INTRODUCTION

IlyanaMushaeva and Amber Moscato

Department of Electrical and Computer Engineering

Temple University

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Spectrometry vs. Spectroscopy

  • Mass spectroscopy is an out of date term for mass spectrometry. Spectroscopy describes measurements involving electromagnetic radiation, such as infrared spectroscopy or ultraviolet spectroscopy.
  • Mass spectrometry involves molecules being analyzed by their fragmentation patterns, not by their interaction with EM radiation.
  • The reason the term mass spectroscopy was used is that the fragmentation patterns of the molecules were originally recorded on a photographic plate, whichinvolved electromagnetic radiation.
  • However, modern mass spectrometers do not use this output method, and so the term mass spectroscopy is no longer correct.
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What is Mass Spectrometry?

  • Measures characteristics of a molecule by converting into ions.
  • A sample is first ionized (proteins in this case).
  • Specific ions are separated according to their mass-to-charge ratio (m/z).
  • Each ion is counted using a detector.
  • Proteinsare a long sequence of 20 Amino Acids
  • Ionization removes charged particles from the molecule.
  • The molecular ion and fragments are formed.
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Mass Spectrum

  • Each “bar” represents an ion with specific m/z and an abundance.
  • The highest mass ion is the Molecular Ion.
  • The Base Peak is the most abundant ion, assigned 100%.
  • All other fragments are percentages of the Base Peak.
  • y-axis: signal intensity of ions
  • x-axis: m/z

Here are simple examples:

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Tandem Mass Spectrometry MS/MS

  • Multiple steps of Mass Spectrometry selection with some form of fragmentation occurring in between the stages.
  • Precursor Ion Scan: monitoring for a specific loss from the precursor ion. The first and second mass analyzers can scan across the spectrum as partitioned by a user defined m/z value. The experiment is used to detect specific parts within unknown molecules.
  • The first scanned is considered the Precursor Ion
  • The second scanned is considered the Product Ion
  • Can be performed in either time or space. For our case, we are dealing with time, which utilizes an Ion Trap (counts/second).
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The Data

Scan 2221 – precursor to 2222

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The Data

ms/ms fragment scan 2222 of mass 747.48

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Brief Bibliography

  • D. Anastassiou, Genomic Signal Processing, IEEE Signal Processing Magazine, 2001.
  • “Introduction to Mass Spectrometry,” available at http://www.chem.arizona.edu/massspec/intro_html/intro.html
  • P.P. Vaidyanathan and Byung-Jun Yoon, The role of signal-processing concepts in genomics and proteomics, Journal of the Franklin Institute, 2004.