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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes - PowerPoint PPT Presentation


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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes. GFP-RAS2 in living Saccharomyces cerevisiae. Images from Roger Tsein’s Lab. Making a Fusion Protein:. Yak1 Gene: DNA: AAAG ATG AATCAG ... ACATCG TGA CCTA... Protein: N- Met-Asn-Gln-…-Thr-Ser-C

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Presentation Transcript
slide5

Making a Fusion Protein:

Yak1 Gene:

DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:

DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP

slide6

Making a Fusion Protein:

    • Yak1 Gene:
    • DNA: AAAGATGAATCAG ... ACATCGTGACCTA...
    • GFP Gene:
    • DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...
  • Fusion Gene:
  • AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...
slide7

Making a Fusion Protein:

Yak1 Gene:

DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:

DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP

N

C

Fusion Gene:

AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

Fusion Protein:

N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C