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Selling SIOdense Products

Selling SIOdense Products. Formerly Proxeon Erik Verschuuren European Sales Manager nHPLC. Matching the proteomics workflow. EASY- nLC TM and EASY-Column™: effortless, split-free nano LC and chromatographic excellence for LC-MS. StageTips TM Microcolumns – fail-safe purification.

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Selling SIOdense Products

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  1. Selling SIOdense Products Formerly Proxeon Erik Verschuuren European Sales Manager nHPLC

  2. Matching the proteomics workflow EASY-nLCTMand EASY-Column™: effortless, split-free nano LC and chromatographic excellence for LC-MS StageTipsTMMicrocolumns– fail-safe purification World-renowned ion sources and emitters to enhance nano ES performance ProteinCenterTM: confident interpretation of complex data – in minutes

  3. Selling EASY-nLC

  4. Routine tool for Proteomic applications • Qualitative ID • Peak widths, throughput, Sensitivity, Ease of Use • Relative Quantitation • + Precision, accuracy (pump and autosampler) • Targeted Quantitation • + Precision, accuracy (pump and autosampler)

  5. What they say about EASY-nLC “...It’s so simple to use, I even installed the second one myself. And their service has been excellent.” John M. Asara, Mass Spectrometry Core, Beth Israel Deaconess Medical Center, Boston, MA. “We are extremely happy with the reliability and reproducibility of the EASY-nLC system.” Kelly Hogue, Lab Manager, Mass Spectrometry (WB) Group, Institute of Molecular and Cell Biology, Singapore “LC-MS experts and biologists alike require trouble-free operation and ease of use along with top performance. Experience has shown that the EASY-nLC system readily meets those high demands.” Professor Matthias Mann, Max Planck Institute for Biochemistry, Martinsried, Germany

  6. EASY-nLC - effortless, split-free nano LC • Unrivalled ease of use • Proven performance with state-of-the-art MS systems • Excellent high resolution results • Easy maintenance, access and service • Easy switching between 1- or 2-column configuration • 2D-enabled operation • identify more proteins than conventional approaches EASY-nLC II Boosting productivity

  7. Stable, pulsation-free gradients from 100 nL/min • Factory-configured flow paths minimize swept volume • Split-free gradient mixing – no flow splitting • Step-controlled syringe pumps – for accurate nano-flow rates • Two flow sensors before high pressure mixing – guarantees accurate delivery of mobile phases

  8. EASY Connections

  9. EASY Method Writing Just, 5 Simple Steps !

  10. EASY (Xcalibur) Driver EASY-nLC can be controlled from Xcalibur or as a stand alone unit without the need for additional computers

  11. Easy maintenance, access and service • Built-in PC & software • automated, built-in maintenance steps • remote access via internet for system interrogation • event log for troubleshooting • Modular design for easy component exchange • Pulsation-free pumps minimize need for replacement of seals, valves and fittings Buffer bottles Switching valves PC and software Dual nano-flow sensors Nanoliter pumps “I love the remote assistance feature with this instrument..” Eric Thomas, Ph.D., Staff Scientist, Proteomics, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida

  12. Remote Support

  13. What’s in the Box Optional

  14. What’s Next.....

  15. What’s Next: UHPLC – EASY-nLC • Nano-flow 1000 Bar (15KPSI) integrated LC system. • Based on existing EASY-nLC 300 Bar design • (conceptually 95% identical) • Same performance but higher pressure • Same ease of use • Same look and feel • Increased robustness • Higher price • Highly competitive • Launch planned for ASMS

  16. The others….

  17. Waters NanoAcquityRT Reproducibility 3 DAYS Waters technical note 10 DAYS Customer reported work

  18. 1200 HPLC Split Nanoflow 100 nL 1000 nL/min Pressure 5880 PSI 1200 HPLC-Chip Chip split less, Still operated by a split HPLC Agilent

  19. NanoLC Ultra Splitlessnanoflow low microflow 50 nL-1000nL / min 1-10 uL/min UPLC 10,000 PSI Temperature control 5˚C – 40˚C Tray cooling Injection 100 nl – 10 uL NanoflexChipLC Temperature control 25˚C - 60 ˚C 4000 PSI 75 um x 15 cm Currently only C18 phase 3 or 5 um 120 or 300 Ǻ Eksigent A mess to set up…

  20. Dionex • Ultimate 3000 RSLCnano system • Splitlessnanoflow microflow • 50 nL 50 uL / min • Modular LC design • UV detector flow cells • Column heater • 5˚C – 70˚C • UPLC • 10,000 PSI • Injection • 20 nL – 25 uL (exchangeable syringes)

  21. Opportunities • NEW LC-MS projects • Replace OLD equipment • LC Packings • Waters CAP-LC • Agilent 1100 and 1050

  22. Thermo Scientific nanoSpray Flex Ion Source • Simple ion generation • Fast and flexible route for ion generation • Designed for simplicity and ease of use • Excellent spray stability • Single set-up for all online nanoflow applications

  23. ProteinCenter is a Productivity Tool for Proteomics ProteinCenter • Overcomes well-recognized, time-consuming challenges • Offers confident interpretation of proteomics data • Reveals the biological context • …of data set comparisons • …of each data set • …in quantitative studies • …for a single protein

  24. Installation of ProteinCenter, Mascot-like • Customer buys an in-house pre-installed ProteinCenter server • Access ProteinCenter through standard web browsers • Database automatically updated weekly ProteinCenter update server Any workstation in lab Customer In-house Server

  25. Brochures

  26. Matching the proteomics workflow EASY-nLCTMand EASY-Column™: effortless, split-free nano LC and chromatographic excellence for LC-MS StageTipsTMMicrocolumns– fail-safe purification World-renowned ion sources and emitters to enhance nano ES performance ProteinCenterTM: confident interpretation of complex data – in minutes

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