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  1. OutReachIdeas •  Emphasize the importance that she spend time learning about all aspects of VB, perhaps focusing on the existing tutorials as a start but not investing any time modifying them as they will ultimately need to be adapted to VB 2..0 •  Examine the possibility that her first overseas training involvement might be a short course in Brazil in association with an already planned meeting (in June?) to introduce and discuss the Rhodnius genome assembly and annotation.  It would be important that an experienced VB member (Dan Lawson?) accompany her on this course. •  Include her on all genome planning calls (e.g., sand flies and the upcoming Anopheles genomes efforts) as an observer initially but ultimately as an active voice representing both VB user community and VB participation as associated with outreach.. •  Plan on Gloria visiting EBI and learning about the outreach efforts of Ensembl staff as well as spending time with EBI, Imperial, Harvard/NM, and Crete VB staff.   • Develop a system for providing single person responsibility for oversight and management of the help requests and other communications with the community. • Develop specific methods for helping the user community:a.  to develop better methods and examples (perhaps in association with training programs) of how to get community data submitted to VB.b.  help community input links between gene IDs in literature with VB and GenBank.c.  develop CAP outreach.d.  get community feedback for improvements.

  2. JarekKrzywinsky • Aim 1: Characterize transcroptsexpessed during development of An. gambiae G3 (16 timepoints) from 20 hour embryos to 20 day old adults. • Aim 2: Discover additional genes induced by following physiological stress: sub-lethal insecticide exposure, sub-optimal relative humidity, and females post blood-feeding

  3. Sex-specific gene expression • Aim 1: We will collect 10-12 h, 20-22 h, 28-30 h, and 36-38 h old embryos; 1st instar larvae 12-14 h after hatching, 2nd and 3rd instar larvae 12-14 h after ecdysis, 4th instar larvae 12-14 h, 22-24 h and 36-38 h after ecdysis; 4 h, 10 h, and 20 h old pupae; and adults at 2, 10, and 20 days post-emergence. • Sexing will be done by PCR, eGFP of a strain that expresses GFP only in females, and by morphology in pupae and adults

  4. Gene Expression following stress • Deltamethrin exposure to LT10 in ideal insectary conditions followed by RNA analysis at 2, 24, 48 and 72 hours post exposure (controls teated to clean test papers) • Mosquitoes exposed to low RH (27C, 60RH) and RNA analysis at 2, 24 48 and 72 hours post exposure (same control) • Blood feeding followed by RNA analysis at 2, 24, 48 and 72 hours post feeding (same control)

  5. Illumina Sequencing (HiSeq2000)

  6. Cate Hill: RAD sequencing of Ixodesscapularis • Aim 1: Discover and map RAD-linked SNP markers in an I. scapularis cross, focusing on parents and progeny of a cross • Aim 2: Use RAD seqeuncing to investigate diversity in up to 10 populations with ~100 larvae per location. Location pools will be barcoded. • Use above data plus physical mapping by FISH to integrate sequence, genetic and physical maps.