GAPDHa Suppresses Heat Shock Induced DNA Fragmentation in Arabidopsis plant 44 C 2h+22 C 24h No treated GAPa:GFP BIP:GFP No treated 44 C 2h A B 44 C 2h + 22 C 24h
GAPDHa Suppresses Heat Shock Induced Cell Death in Arabidopsis Plant untreated DAPI stained cell (%) treated GAPa:GFP BIP:GFP
Heat Shock Induces ROS Accumulation in the Arabidopsis Plant + heat shock - heat shock 64 Counts 32 0 32 64 96 128 160 192 224
GAPDHa Suppresses ROS Accumulation Induced by Heat Shock stress Bright Rh123 untreated treated E
Nucleoside diphosphate kinase (NDPK) • a hexameric protein composed of 17kDa monomer • catalyze the reversible transfer of the 5’-terminal phosphates from triphosphate nucleosides (or their deoxy derivatives) to nucleoside diphosphates to generate NTPs or dNTPs • in animals, NDPKs have important role in control of cell proliferation, regulation of transcription, and protein phosphotransferase activity • - in plants, it is associated with phytochrome B response, UV-B signaling, heat stress and hormone related responses • - very little is known about the nature of regulation of NDPK gene in eukaryotes.
Measurement of Redox-State in the Cell Using Dichlorofluorescein Diacetate (DCFH-DA) DCFH-DA Cell DCFH H2O2 DCF fluorescence
YNK Regulates ROS Generation w.t. Dynk % DCF stain F P L w.t. 7.0 3.5 89.9 Dynk 10.7 36.0 53.3
YNK Regulates ROS Generation Dynk1 w. t. Bax FL1, Rhodamin123 ; FL2, Propidium iodide