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α 1

Sm-AMP-D1. MusaDef1. MusaDef2. MusaDef3. MusaDef4. conserved γ-core motif (GXCX 3–9 C). α 1. α 1. β 2. β 2. β2 - β 3 loop. β 3. β 3. β2 - β 3 loop. β 1. β 1. α 1. α 1. β 2. β 2. β 3. β 3. β2 - β 3 loop. β2 - β 3 loop. β 1. β 1.

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α 1

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  1. Sm-AMP-D1 MusaDef1 MusaDef2 MusaDef3 MusaDef4 conserved γ-core motif (GXCX3–9C) α1 α1 β2 β2 β2-β3 loop β3 β3 β2-β3 loop β1 β1 α1 α1 β2 β2 β3 β3 β2-β3 loop β2-β3 loop β1 β1 Supplementary figure S1: Alignment of Sm-AMP-D1 mature sequence with four most closely related banana defensin-like protein sequences. All eight cysteines are conserved as is the γ-core motif (GXCX3–9C). Structures of the four banana defensin like proteins show basic similarities with other potent antifungal defensins. MusaDef1 MusaDef2 MusaDef3 MusaDef4

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