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Lab 6: Molecular Biology

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  1. Lab 6: Molecular Biology • Description • Gel electrophoresis • cut DNA with restriction enzyme • fragments separate on gel based on size

  2. Lab 6: Molecular Biology • INTRODUCTION: • restriction enzyme • gel electrophoresis • DNA is negatively charged • smaller fragments travel faster • RFLPs • DNA Fingerprint

  3. Hypothesis • IND VAR: DNA samples • DEP VAR: RFLP fragment sizes • PREDICT: who is the criminal?

  4. Lab 6: Gel Electrophoresis • Data and analysis DNA = negatively charged correlate distance to size smaller fragments travel faster & therefore farther

  5. DATA • Sketch of gel • Data table from pg. 43 • Logarithmic graph.

  6. Analysis • Answer “interpretation of results” on p. 46

  7. Questions • Answer all questions asked DURING THE LAB at end of lab. • Pp. 29, 34, 36