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Optogenetics

Optogenetics

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Optogenetics

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  1. Optogenetics Use of optics & Use of genetics http://www.livescience.com/29340-optogenetics-brain-research-breakthrough-nsf-bts.html

  2. Transgenic approaches are easy with fruit flies www.domyownpestcontrol.com

  3. How could we target ChR to motor neurons? OK371-Gal4 x UAS-ChR2 In other words, what gene is OK371?

  4. Human Brain https://en.wikipedia.org/wiki/Serotonin

  5. Adult Drosophila brain with neurons stained green and the neurotransmitter serotonin stained red.

  6. Green are serotonin containing neurons http://www.mdpi.com/1422-0067/10/2/407/htm

  7. Experiments • Larva • Motor neurons • Examine acute behavioral changes

  8. Mechanism of Channelrhodopsin2 in motor neurons Channelrhodopsin2 inactivated (normal light) Na+ Ca2+ ChR2 NO Muscle contraction

  9. Mechanism of Channelrhodopsin2 in motor neurons Channelrhodopsin2 activated (blue light) Bluelight Na+ Ca2+ ChR2 Glutamate Glutamate instead of Acetylcholine in Drosophila Muscle contraction

  10. Blue light source setup

  11. Bodywall contractions in larva

  12. Group-1

  13. Neuromuscular Junction ES Presynaptic terminal Synaptic cleft Postsynaptic target Glutamate Receptors

  14. What would happen with the Minis at fly NMJ with ChR2 activation?

  15. Lets do it !