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实验二 人类染色体核型分析

实验二 人类染色体核型分析. 一、实验目的 通过实验掌握染色体核型分析的常用方法以及 G 带的带型特征 和识别技巧，初步学会识别 G 带染色体。 二、实验原理 将一个细胞内的染色体按照一定的顺序排列起来构成的图像就 称之为该细胞的 核型 (karyotype) 。核型分析有多种方法，如 G 带， R 带、 C 带等。将染色体标本用显带方法处理后，再用 Giemsa 染 色，这类技术就称为 G 分带，通过显微摄影，就可得到 G 带染色体 的显微相片。. Chromosome classification

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实验二 人类染色体核型分析

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  1. 实验二 人类染色体核型分析 一、实验目的 通过实验掌握染色体核型分析的常用方法以及G带的带型特征 和识别技巧，初步学会识别G带染色体。 二、实验原理 将一个细胞内的染色体按照一定的顺序排列起来构成的图像就 称之为该细胞的核型(karyotype)。核型分析有多种方法，如G带， R带、C带等。将染色体标本用显带方法处理后，再用Giemsa染 色，这类技术就称为G分带，通过显微摄影，就可得到G带染色体 的显微相片。

  2. Chromosome classification lRelative length : Single / total ( haploid set ) lArm index : q : p lCentromere index : p / single ISCN: an international system for human cytogenetic nomenclature

  3. Group A Chromosome 1, 2 and 3 Group B Chromosome 4 and 5 Group C Chromosome 6 to 12 and the X Chromosome Group D Chromosome 13 to 15 ( the “large acrocentrics” satallited short arms ) Group E Chromosome 16 to 18 Group F Chromosome 19 to 20 Group G Chromosome 21 and 22 ( the “small acrocentrics” satallited short arms ) and the Y Chromosome

  4. 三、实验步骤 1．先贴一张相片于报告纸上方。 2．将另一相片上的染色体逐个剪下，按丹佛和人类染色体遗传学命名的国际体制（ISCN）排列编号。粘贴时短臂向上，长臂向下。 3．在分析结果中，写出该细胞的核型式，注明性染色体。

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