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This document provides a detailed collection of primer sequences for c-Fos and FosB, crucial for molecular biology experiments. Each primer is labeled with its specific region, including E1U to E5L and I3L to I4L, along with detailed nucleotide sequences. Additionally, molecular weight markers are included to aid in the verification of PCR products. This resource serves as a valuable tool for researchers studying gene expression and signaling pathways associated with c-Fos and FosB, enhancing experimental efficiency and accuracy.
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A) 551 843 1598 1849 2255 2362 2480 3967 c-fos E1U E2U E3U E4L E2L E3L I3L 27827 27293 25472 25152 24696 24589 23328 23172 23033 20945 fosB E5L E4U I4U I4L B) 5’ end c-fos primers E1U 815 5’-CCAGCATGGGCTCTCCTGTCAACACA E2U 1641 5’-CCACGGTGACAGCCATCTCCACCA E2L 1747 5’-TCCGTAAGGATGGGGCGCTCTGGT E3L 2317 5’-GGCTGCAGCCATCTTATTCCGTTCCC E3U 2324 5’-CGGAATCGGAGGAGGGAGCTGACAGATA I3L 2440 5’-ATGCTCAAGGACCCTGCGCCCATAGT E4L 2581 5’-TCGGTGGGCTGCCAAAATAAACTCCAGT fosB primers E4U 23225 5’-CGGGCTGCAAGATCCCCTACGAAGA I4U 23178 5’-GCCGAGGTGAGAGATTTGCCAGGGTC I4L 23050 5’-AGCCGTCAGGTTGGGGGGTGC E5L 22951 5’-GACCTCCGGGCAGGTGAGGACAAAC C) c-fos fosB MW marker E1U-E2L E2U-E3L E3U-E4L E3U-I3L E4U-E5L I4U-I4L MW marker nt 300 200 100
