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Explore the intricate world of gene prediction and transcriptional units in both prokaryotes and eukaryotes, including the splicing process and promoter sequences. Learn about consensus sequences and codon usage tables for human genes. Delve into the fascinating world of gene identification.
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Chapter 6 Gene Prediction: Finding Genes in the Human Genome
Figure 6.2: Schematic view of a typical prokaryotic gene and the mRNA transcribed from the gene
Figure 6.3: Transcriptional units in prokaryotes (A) can contain multiple open reading frames; eukaryotic transcriptional units (B) can have only one ORF each
Figure 6.4: The eukaryotic promoter, defined by an assemblage of interacting protein transcription factors bound to DNA
Figure 6.5: A eukaryotic mRNA is processed by splicing after transcription to remove introns and join exons into a continuous coding sequence
Figure 6.6: Consensus promoter sequences based on a few strong E. coli promoters
Figure 6.7: The six possible reading frames within a short piece of double-stranded DNA
Figure 6.8: Output from NCBI’s ORF Finder program Courtesy of ORF/National Center for Biotechnology Information
Figure 6.T1: Promoter sequences recognized by some E. coli sigma factors
Figure 6.9: The splicing process and consensus sequences at the exon-intron boundaries and branch site
