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Supplementary Figure 1. A. 827 + EGF/bFGF. 827 + EGF/bFGF. 827 + EGF/bFGF. 0. PHA665752. SU11274. SGX523. -1. Proportion of wells w/o sphere (log 10 ). -2. -3. -4. 0. 50. 100. 150. 200. 0. 10. 20. 30. 40. 50. 0. 5. 10. 15. 20. 827 + HGF. 827 + HGF. 827 + HGF. 0.

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Presentation Transcript
slide1
Supplementary Figure 1

A.

827 + EGF/bFGF

827 + EGF/bFGF

827 + EGF/bFGF

0

PHA665752

SU11274

SGX523

-1

Proportion of wells

w/o sphere (log10)

-2

-3

-4

0

50

100

150

200

0

10

20

30

40

50

0

5

10

15

20

827 + HGF

827 + HGF

827 + HGF

0

PHA665752

SU11274

SGX523

-1

Proportion of wells

w/o sphere (log10)

-2

-3

-4

0

50

100

150

200

0

20

40

60

80

100

0

20

40

60

80

100

number of plated cells

B.

131 + EGF/bFGF

131 + EGF/bFGF

131 + EGF/bFGF

0

PHA665752

SU11274

SGX523

-1

Proportion of wells

w/o sphere (log10)

-2

-3

-4

0

100

200

300

400

500

20

40

60

80

100

0

0

50

100

150

200

131 + HGF

131 + HGF

131 + HGF

0

PHA665752

SU11274

SGX523

-1

Proportion of wells

w/o sphere (log10)

-2

-3

-4

0

50

100

150

200

0

50

100

150

200

0

50

100

150

200

number of plated cells

slide2
Supplementary Figure 2

464T

+EGF/bFGF

464T

+EGF/bFGF

-SU11274

464T

+EGF/bFGF

+SU11274

2.08%

31.8%

25.4%

CD133-APC

Isotype-APC

464T

+HGF

464T

+HGF

-SU11274

464T

+HGF

+SU11274

Isotype-APC

2.83%

CD133-APC

9.00%

7.53%

slide3
Supplementary Figure 3

GFP

ABC

Merge

Merge + DAPI

Field 1

GFP

ABC

Merge

Merge + DAPI

Field 2

GFP

ABC

Merge

Merge + DAPI

Field 3

slide4
Supplementary Figure 4

mCMV backbone reporter

mCMV-TCF/LEF reporter

464T

Relative Luciferase Unit

Wnt3a

(ng/ml)

0

25

50

slide5
Supplementary table 1

Expression levels of WNT target genes in METhigh/+ cells

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