Preimplantation Genetic Testing (PGT) at Hardik IVF

1. Preimplantation Genetic Testing (PGT) at Hardik IVF: Your Path to Healthier Embryos and Higher IVF Success Introduction For couples who are undergoing the process of In-Vitro Fertilization (IVF) One crucial issue is how to increase the odds of having an uninjured pregnancy and a healthy baby. Preimplantation Genetic Test(PGT) offered by the Hardik IVF and Fertility Center provides the ability to test embryos for chromosomal and genetic issues prior to the transfer. By identifying only healthy embryos PGT lowers the chance of miscarriage and improves the rate of implantation, and helps prevent transmission of genetic disorders. This article will explain the basics of what PGT is, the different kinds, the steps to follow in Hardik IVF, and the reasons why it could be the best option for your family-building journey.

2. What Is PGT? PGT stands for Preimplantation Genetic Testing. (PGT) is the various diagnostic methods that are applied to embryos born through IVF prior to being placed into the uterus. In the simplest sense, PGT answers the question What embryos are free of obvious genetic or chromosomal defects? Only "healthy" embryos are selected to transfer, providing: ● Risk of miscarriage is reduced, as chromosomal mistakes are the leading reason for early loss. ● Higher rates of implantation, due to the embryos that are chromosomally normal are more stable in implantation. ● Preventing the inheritance of diseases, ensuring that children will not be exposed from diseases with a single gene. ● The Hardik IVF clinic is where PGT integrates seamlessly with the IVF cycle, combining the latest genetics with compassionate care for the patient. Types of PGT Hardik IVF provides three main PGT services: 1.PGT-A (Aneuploidy Screening) ○ Examine embryos for abnormal numbers of chromosomes (too numbers of or too little chromosomes). ○ Aneuploid embryos are often unable to implant, or cause miscarriage. 2.PGT-M (Monogenic/Singe-Gene Defect Testing) ○ Tests for inherited conditions that are specific to the patient like cystic fibrosis as well as thalassemia and Huntington's disease. ○ Perfect for couples who carry a gene responsible for causing disease.

3. 3.PGT-SR (Structural Rearrangement Analysis) ○ Detects large chromosomal rearrangements--translocations, inversions--that can disrupt embryo development. ○ The value is in the event that one partner is able to achieve a balanced rearrangement, and also the there is a risk of embryos that are not balanced. Each test addresses distinct genetic risk factors.The best Hardik IVF consultant will suggest the right PGT type according to your family and personal genetic background. Who Benefits from PGT? PGT is particularly useful to: ● Women over 35: Age-related eggs quality decline can increase the rate of aneuploidy. ● Recurring IVF errors or miscarriages Chromosomal error is the most frequent cause. ● Genetic carriers known to be present: Couples who carry recessive or dominant disease genes. ● Chromosomal rearrangement carriers Inversions or balanced translocations with one of the partners. ● Need for a single-embryo single-blastocyst transfer and minimizes the risk of multiple pregnancies. transfer PGT-A permits safe While PGT costs money and takes one or two days of lab time, the chance to prevent a failed cycle or miscarriage usually outweighs these concerns. The PGT Process at Hardik IVF Hardik IVF's team of multidisciplinary specialists makes sure your PGT experience is seamless and dependable. This is a step-by-step guideline: 1.Pre-Cycle Genetic Counseling

4. ○ Assessment: Examine your medical, obstetrical, and family background. ○ Risk Identification: Determine the PGT test(s) best suit your requirements. ○ Informed Consent: Describe the benefits and limitations, timelines and the costs. 2.IVF Stimulation & Egg Retrieval ○ Standard ovarian stimulation produces multiple eggs. ○ Mature eggs are taken using ultrasound, and the use of sedation. 3.Fertilization & Embryo Culture ○ Eggs are fertilized using sperm using ICSI (if the procedure is). ○ The embryos are cultivated until the blastocyst stage (day 5-6) at which point they contain 100+ cells as well as distinct trophectoderm. 4.Embryo Biopsy ○ A small number of outer cells (trophectoderm) are removed gently with a laser-assisted biopsy. ○ The embryos that have been biopsied remain in the culture until analysis is completed. 5.Genetic Analysis ○ Next-Generation Sequencing (NGS) or microarray techniques are utilized in Hardik IVF's Genetics Lab. ○ Results are available in 24 to 48 hours. They will report aneuploidy monogenic disorders or structural anomalies. 6.Embryo Selection & Transfer ○ The embryos that are healthy are chosen to be used for the uterine transfer. ○ Two or three embryos are placed inside the uterus. Any unaffected blastocysts can be frozen. 7.Follow-Up and Pregnancy Test ○ Progesterone assistance and monitoring continues.

5. ○ A blood b-hCG test 10-12 days post-transfer confirms pregnancy. All through, Hardik IVF's patient coordinators are on the lookout for prompt information, support for emotional issues and logistical support. Benefits of PGT at Hardik IVF ● Superior Laboratory Standard: Our laboratory has the most modern NGS platforms as well as skilled technologists. ● Integrative Care: Fertility specialists as well as geneticists and embryologists collaborate to achieve optimal results. ● Customized Protocols: Stimulation as well as biopsy techniques that are customized to each patient's specific health his/her history. ● Lower Cost Over Time: A lower number of unsuccessful cycles and miscarriages may reduce the overall emotional and financial burden. ● Counseling for Emotional Support: A dedicated counselor assists couples in making difficult decisions and deal with the results. Patients of Hardik IVF routinely report greater confidence and less anxiety when PGT is a included in their IVF program. Limitations & Considerations ● While PGT provides clear benefits but it's essential to be aware: ○ There is no guarantee: Even embryos that are euploid may do not implant. ○ Cost Addition PGT generally increases NPR 30 000 every cycle to Nepal. ○ Biopsy Risks: Very low risk of causing damage to an embryo. However, the latest techniques limit that to 1-2 percent. ○ Ethics: Deciding how to do with the affected embryos will require reflection and personal reflection.

6. ● The Hardik IVF team will help you evaluate these elements and make informed decisions aligned with your personal values. Real‑World Impact: Patient Stories Case 1: Recurrent Miscarriage A couple who had suffered 3 miscarriages prior to their first attempt had to undergo IVF with PGT-A in Hardik IVF. They transferred one embryo that was euploid and had an embryo that was healthy on their first PGT cycle - avoiding the pain of another loss. Case 2: Genetic Carrier Couple Both parents are carriers of beta-thalassemia genes. They chose to test for PGT-M choosing embryos without the condition. Their daughter, who is now two years old, is still well and pain-free due to the early screening of genetics. Costs of PGT At Hardik IVF and Fertility Center, typical PGT pricing in Nepal is: ● PGT‑A: NPR 30,000 per cycle ● PGT‑M: NPR 2,50,000–4,00,000 per targeted gene ● PGT‑SR: NPR 2,50,000–4,00,000 per cycle These fees cover biopsy, genetic analysis, and reporting. Hardik IVF offers flexible payment plans and can bundle PGT with IVF packages to reduce overall expense. Conclusion Preimplantation Genetic Testingcan change IVF from a hopeless step into a more knowledgeable strategy-based approach, especially for patients with a high risk of genetic or age-related. Hardik IVF and Fertility Center, PGT is performed with the highest technological standards and a compassionate approach to patient care.If you're concerned about aneuploidy, single-gene disorder or chromosomal rearranged

7. chromosomes, our team of experts will help you through each step of the process: from genetic counseling through embryo transfer. If you choose to use to undergo PGT throughbest Hardik IVF centerYou take the first step to build an enviable family and maximizing the chances of achieving IVF results while reducing your the financial and emotional strain.