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Introduction to the LSR II and FACSDiva 6.0 Software

Introduction to the LSR II and FACSDiva 6.0 Software. Four-laser, 10-color benchtop flow cytometer . side door. left cover. right cover. High Throughput Sampler . fluidics interconnects. control panel. SIP. power switch. Laser: 488nm, 355nm, 405 nm, 633 nm . Control Panel.

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Introduction to the LSR II and FACSDiva 6.0 Software

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  1. Introduction to the LSR II and FACSDiva 6.0 Software

  2. Four-laser, 10-color benchtop flow cytometer side door left cover right cover High Throughput Sampler fluidics interconnects control panel SIP power switch Laser: 488nm, 355nm, 405 nm, 633 nm

  3. Control Panel Sample flow rates: approximately 12, 35, and 60 μL/min ( LO, MED, and HI settings ) SAMPLE FINE ADJ LO MED HI RUN STNDBY PRIME sample flow rate fluid control buttons fluid control buttons

  4. Octagon Detector Arrays longpass dichroic mirrors bandpass filters 355 nm (UV) : Indo-1, DAPI, Alexa Fluor 350 405 nm (Violet) : CFP, Alexa Fluor 430, Pacific Blue, Marina Blue, Alexa Fluor 405 488 nm (Blue) : FITC, PE, PerCP, Pe-Cy5 (aka CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas Red 633 nm (Red): APC, APC-Cy7, Alexa Fluor 647, Alexa 680

  5. 488-nm blue laser 633-nm red laser PerCP-Cy5.5 695/40BP 685LP PerCP PI PE-Cy5 PE-Cy5 APC 670/14 660/20 FITC 635 LP 530/30 GFP 505 LP 488/10 SSC 735 LP 735 LP 575/26 780/60 780/60 PE PE-Cy7 APC-Cy7

  6. 355-nm UV laser 405-nm violet laser Alexa405 DAPI CascadeB Pacific Blue Alexa350 440/40 450/50 505 LP 505 LP 525/50 530/30 AmCyan CascadeY Indo-1 (Blue)

  7. To Login the computerLogin Name: flow user Password: fccf To start the software, double click the shortcut icon on the desktop shortcut icon Login to FACSDiva Software

  8. FACSDiva workspace

  9. Preparing Sheath and Waste Containers Fluidics Cleaning Each time you finish using the cytometer, clean the sample injection tube and the area between the injection tube and the outer sleeve. This prevents the sample injection tube from becoming clogged and removes dyes that can remain in the tubing. CLEANING INSTRUCTIONS ARE POSTED ON THE LSRII

  10. LSRII Location: BioSquare III, 670 Albany St. 5th Floor (Card access is required to enter the room) FCCF web address: http://www.bu.edu/cores/flow-cytometry FCCF email: flowcore@bu.edu Sorting facility phone number: 617-414-5225

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