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Dynamic Light Scattering

Dynamic Light Scattering. Outline. Measurement system Autocorrelation functions Coulter N4Plus. DLS Measurement System. Photon Counting. photon counts per time. µ. Light intensity. Autocorrelation Functions. delay time. Change the abscissa to a long scale:.

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Dynamic Light Scattering

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  1. Dynamic Light Scattering

  2. Outline • Measurement system • Autocorrelation functions • Coulter N4Plus

  3. DLS Measurement System

  4. Photon Counting photon counts per time µ Light intensity

  5. Autocorrelation Functions delay time Change the abscissa to a long scale:

  6. Averages of the Autocorrelation Functions ensemble average = long-time average ergodicity average over all possible configurations of particle positions measured DLS

  7. Two Ways to Estimate the Baseline (average intensity)2 vs. last-channel data in The difference is the baseline error.

  8. Normalized Autocorrelation Function decays to zero. coherence factor baseline-subtracted, normalized intensity autocorrelation function

  9. g2(t) and g1(t) Electric-field autocorrelation function normalized electric-field autocorrelation function

  10. Autocorrelation Function in Various Scales

  11. Coulter N4 Plus particle size range: 3 nm to 3 µm temperature: 0 to 90 °C automatic or manual selection of Dt automatic or manual pre-scaling of photon counting size distribution analysis by CONTIN (Provencher)

  12. Autocorrelator in N4 Plus

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