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by Ivo Frébort

Protein electrophoresis. by Ivo Frébort. Polyacrylamide chemistry. S 2 O 8 2-  2 SO 4 -. TEMED ( N, N,N´,N´- tetramethylethylendiamin). polyacrylamide. acrylamide 3-15 %T. N,N ’ -methylene-bis-acrylamide 5 %C. Gel preparation. Protein visualization. Comassie blue stainig.

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by Ivo Frébort

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  1. Protein electrophoresis by Ivo Frébort

  2. Polyacrylamide chemistry S2O82- 2 SO4-. TEMED ( N, N,N´,N´-tetramethylethylendiamin) polyacrylamide acrylamide 3-15 %T N,N’-methylene-bis-acrylamide 5 %C

  3. Gel preparation

  4. Protein visualization Comassie blue stainig Silver stainig Sensitivity 10-50 ng/band Sensitivity 500 ng/band

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