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Investigating HIV-1 p6 (31-46) binding to Alix at residues 31I.D.K.E.L.Y.P.L..L.R..L. and its impact on relative b-Gal activity. Mutational analysis includes p6 ALIX, DPRR, and various p6 p9 combinations.
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A HIV-1 p6 (31- 46) constructs Binding to Alix 31I D K E L Y P L T S L R S L F G46 A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A - - - - - - - - - - - - - - - - A Binding motif . . . E L Y P L . . L R . L . . Relative b-Gal activity 0 0.75 0.25 0.50 1.00 Relative b-Gal activity B 0.75 0 0.25 0.50 1.00 1.25 1.50 p6 ALIX DPRR D716-768 D541-582 D541-582 V509A D541-582 F676D p9 p6 p9 p6 p9 p6 p9 p6 p9