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Comparison of Excitation Output for Hg vs HgXe vs Xe Lamp with Optisplit in TE200

This Norton group meeting from 2010 discusses the comparison of excitation output from different lamps using Optisplit in TE200 microscope. It also highlights the necessity of 2" optics to efficiently couple Xe lamp to the o-split scope.

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Comparison of Excitation Output for Hg vs HgXe vs Xe Lamp with Optisplit in TE200

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  1. Norton group meeting 11/2010, David Neff Comparison of excitation output Hg vs HgXe vs Xe lamp at objective of TE200 with Optisplit:justification for 2” optics necessary to efficiently couple Xe lamp to o-split scope

  2. No lamp (ambient, lights off in room but fluor lights in main area leaking in through window) HgXe lamp Hg lamp this is the output straight from the lamp this is the output straight from the lamp no lamp Input optics on HgXe lamp are inferior so do not directly compare absolute intensities. Relative intensities at different wavelength are valid. position 1 osplitex 470/40&550/20bs 520/40&590/60 em 540LP-green 520/40 -red 595/50 position 4 GFPex 470/40bs 495LP em 525/50 position 2 rhodex 560/40bs 595LP |em 630/60

  3. Xe lamp No lamp (ambient, room lights off but light leak in window from main room) this is the output straight from the lamp no lamp Input optics on HgXe and Xe lamp are inferior so do not directly compare absolute intensities. Relative intensities at different wavelength are valid. position 1 osplitex 470/40&550/20bs 520/40&590/60 em 540LP-green 520/40 -red 595/50 position 4 GFPex 470/40bs 495LP em 525/50 position 2 rhodex 560/40bs 595LP |em 630/60

  4. In the scope: • 59004x dual-band exciter • 59004bs polychroic mirror • In the Optosplit: • ET515/30m for GFP • ET595/50m for rhodamine • T540LPXR

  5. biorad mrc1024 notice all the contrast in the Leica images almost make them look 'worse'. leica sp5 20x 100x

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