1 / 28

Ca 2+ release mechanisms in cardiac & skeletal muscle

Investigate differences in Ca2+ release mechanisms between cardiac and skeletal muscle cells using chimeric proteins and FRET analysis. Explore voltage-sensitive Ca2+ channel characteristics, molecular structures, and genes. Analyze SR Ca2+ release channels, RyR molecular arrangements, and ligand-gated Ca2+ channels. Examine Ca2+ release dynamics during cell shortening and discuss findings.

gili
Download Presentation

Ca 2+ release mechanisms in cardiac & skeletal muscle

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Ca2+ release mechanisms in cardiac & skeletal muscle OBJECTIVES Methodology: Chimeric proteins FRET Differences in Ca2+ release between cardiac and skeletal muscle

  2. INTRODUCTION

  3. Ca2+ channel characteristics

  4. Voltage sensitive Ca2+ channel molecular structure

  5. Mammalian Ca2+ channel genes

  6. SR Ca2+ release channel – Ryanodine receptor (RyR)

  7. RyR molecular structure

  8. Mammalian ligand-gated Ca2+ channel genes

  9. DHPR-RyR molecular arrangment

  10. Ca2+ release in skeletal muscle

  11. Ca2+ release in cardiac muscle

  12. DISCUSSION

  13. Cardiac sequence Skeletal muscle sequence pCac6 (Sk) 1.5 nA pCARD1 15 nA 10 nA

  14. CELL SHORTENING Cardiac sequence Skeletal muscle sequence pCac6 (Sk) pCARD1 Ca2+ + 0 / Cd2+

  15. I II III IV II-III LOOP

  16. Serine687 to alanine (S687A) Serine – PKA (only in Sk)

  17. I II III IV II-III LOOP

  18. Region with RyR-2 cDNA injection Dyspedic myotubes Ca2+ transient during spontaneous contractions Normal myotubes Dyspedic myotubes with RyR-2

  19. Normal myotubes Dyspedic myotubes with RyR-2

  20. Ca2+ current & [Ca2+]i RyR-2 expressing Dyspedic myotubes Ca2+ current RyR-2 expressing Dyspedic myotubes

  21. Ca2+ current CSk3 expressing Dyspedic myotubes Ca2+ transient Dyspedic myotubes Electrically evoked Spontaneous Failure (electrical)

More Related