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Collaborative Research in Biophysics: A Journey of Discovery

Explore the journey of collaborative research in biophysics from 1980 to 2003, encompassing a wide array of animal models, physiological measurements, NMR spectroscopy technologies, and worldwide collaborations. Witness the evolution of research methodologies and the connections made with researchers worldwide to advance understanding of various organ systems and pathophysiological conditions.

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Collaborative Research in Biophysics: A Journey of Discovery

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  1. BC and Me – 1980 to 2003 • 1980: First Meeting –NMR meeting • 1984: Joined Brit’s lab • 1984-87: Animal Models • Open Chest Animals: dog and cat • Volume & pressure overload; ischemia; hypoxia • Closed Chest animals: dog • Volume & pressure overload; cardiomyopathy; heart failure; ischemia • Other intact organs: Skeletal Muscle; liver, brain • Increase work load; ischemia • 1987-2003: Isolated organs, cells & mitochondria

  2. Multiple Technologies • NMR: 31P, 23Na; 1H; 13C; 19F • NADH Fluorescence • NIR Spectroscopy • Other physiological measurements • Piezoelectric crystals: Mechanical function • Laser Doppler: flow in blood vessels; tissue perfusion • Tissue electrodes: Cae2+, Ke+, Nae+ • ECG, EEG • BP, HR, LVP

  3. World Wide Colleagues • British Connection: John Haselgrove • Chinese Connection: Daning Zhang; DJ Wang • French Connection: Bertrand Autlier; Suzanne Wehrli • German Connection: Manfred Prammer • Hungarian Connection: Laci Ligeti; Tomas Ivanics; Aristied Kovach: Kati Megyeri; Laci Deszi • Indian Connection: Hari Subramanian; Ramin Mitra • Israeli Connection: Avraham Mayevsky; Myer Shinnar • Japanese Connection: Shoko Nioka; K Niibori • Netherland Connection: Hans van Beek • Polish Connection: Maria Erecinska; Krzysztof Wroblewski • Russian Connection: Andre Savchenko • Ukrainian Connection: Nicola Doliba; Natalya Doliba; Andriy Babsky • Uruguayan Connection: Juan Crestanello • US Connection: too numerous to list • The World: too numerous to list

  4. NMR Spectroscopy – Multiple collaborations • Intact in vivo brain • Closed Chest Animals: Dog; Cat • Open Chest animals: Dog; Cat • Perfused Heart • Superfused Cardiomyocytes; hepatocytes • Superfused Mitochondria – heart, liver

  5. Closed Skull Cat Brain

  6. Closed Skull Cat Brain – 31P

  7. Open Chest Animal: Dog or Cat

  8. Open Chest Dog – 31P

  9. Closed Chest Dog – 31P

  10. Langendorff Perfused In NMR

  11. Isolated Perfused heart – 31P

  12. Superfusion SystemIsolated Cells & Mitochondria

  13. Isolated Superfused Cardiomyocytes -31P

  14. Isolated Superfused Mitochondria- 31P

  15. Optical Spectroscopy&Electrode System • NADH Fluorescence • Heart • Brain • NIR • Oxygenation • Multiple electrode system • Ca2+ • K+ • Na+

  16. NADH Fluorescence & NMR

  17. NADH FluorescenceHypoxia & NE Administration

  18. Multiparameter Analysis: Flow probe; NADH light guide; Balloon occluder

  19. Multiparameter Analysis: Multi-channel Light Guide; Electrodes (Cae+, Ke+, Nae+; Laser Doppler Tissue Flow; CA flow Probe

  20. Multiparameter Analysis:NADH, Laser Doppler Flow; Ca2e+, K2+

  21. Not the End – Just the Beginning • The story continues through BC’s • Many students • Former colleagues • Friends

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