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Strawberry DNA Extraction. DNA: Basics. D eoxyribo n ucleic a cid Genetic material Chain of molecules linked together DNA contains instructions for traits Wrapped tightly around “histones” Forms chromosomes Housed in nucleus. DNA: Basics. DNA: Structure. Double-helix (twisted ladder)

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Presentation Transcript
dna basics
DNA: Basics
  • Deoxyribonucleic acid
  • Genetic material
    • Chain of molecules linked together
    • DNA contains instructions for traits
  • Wrapped tightly around “histones”
  • Forms chromosomes
  • Housed in nucleus
dna structure
DNA: Structure
  • Double-helix (twisted ladder)
  • Sugar and phosphate backbone
  • Nitrogen bases
  • Subunit: “nucleotide”
dna replication
DNA: Replication
  • Special pairing between nitrogen bases
  • New strand is copy of old strand
dna extraction step 1
DNA Extraction: Step 1
  • Break the cells open (using force)
dna extraction step 2
DNA Extraction: Step 2
  • Dissolve the cell membrane and nuclear envelope (using detergent/soap)

Current Protocols in Protein SciencePublished by John Wiley & Sons, Inc.

dna extraction step 3
DNA Extraction: Step 3
  • Remove proteins that are bound to DNA (using salt to dissolve them)
dna extraction step 4
DNA Extraction: Step 4
  • Precipitate DNA (with ethanol)
dna extraction why
DNA Extraction: Why?

VS.

Species identification