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LIFE13 ENV IT 000461

LIFE13 ENV IT 000461. Polifenoli estratti dai sottoprodotti e reflui agricoli come biostimolanti e prodotti per la difesa: un’importante opportunità per ridurre i rifiuti e stimolare l’economia circolare Prof.ssa Annalisa Romani – Phytolab-DiSIA , Università di Firenze.

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LIFE13 ENV IT 000461

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  1. LIFE13 ENV IT 000461 Polifenoli estratti dai sottoprodotti e reflui agricoli come biostimolanti e prodotti per la difesa: un’importante opportunità per ridurre i rifiuti e stimolare l’economia circolare Prof.ssa Annalisa Romani – Phytolab-DiSIA, Università di Firenze DIFESA INNOVATIVA DELLE PIANTE DAI LORO PATOGENI, NEL RISPETTO DELL’AMBIENTE E NELL’AMBITO DELL’ECONOMIA CICLICA Accademia dei Georgofili, Firenze – 13 novembre 2015

  2. EU project LIFE13 ENV IT 000461 / “EVERGREEN” “Environmentally friendly biomolecules from agricultural wastes as substitutes of pesticides for plant diseases control” Accademia dei Georgofili, Firenze – 13 novembre 2015

  3. TIMETABLE

  4. CIRCULAR ECONOMY A circular economy is one that is restorative and regenerative by design, and which aims to keep products, components and materials at their highest utility and value at all times, distinguishing between technical and biological cycles. Accademia dei Georgofili, Firenze – 13 novembre 2015

  5. BIOMASS AND WASTE INNOVATIVE GREEN REUSE OR EXTRACTIONS TRADITIONAL USE CIRCULAR ECONOMY DiSCARD DISPOSAL OR COMBUSTION NATURAL ACTIVE COMPOUNDS AND ENERGY

  6. THE PROCESS 1 TRACED SOURCE 8 2 STANDARDIZED EXTRACTS 9 REVERSE OSMOSIS & NANO-FILTRATION FEED FIBER (1% TANNINS) MULCHING PRE-TREATMENTS 5 7 4 6 3 WATER EXTRACTION

  7. Accademia dei Georgofili, Firenze – 13 novembre 2015

  8. SweetChestnutfractions: quali-quantitativecharacterization Accademia dei Georgofili, Firenze – 13 novembre 2015

  9. SweetChestnutfractions: quali-quantitativecharacterization Single compounds qualitative analysis of the Sweet Chestnut liquid commercial fraction (fraction 6). Rt=retention time (min); λmax=maximum absorbance wavelength (nm); sh = shoulder. m/z of the most abundant ESI-MS ion is underlined. Accademia dei Georgofili, Firenze – 13 novembre 2015

  10. SweetChestnutfractions: antioxidant and antiradicalactivities Accademia dei Georgofili, Firenze – 13 novembre 2015

  11. Antimycoticactivity • Pomegranate peel (Punica granatum L.); • Sweet Chestnut frac. 6(Castanea sativa Mill.); • C. Myrtle leaves (Myrtus communis L.).

  12. The antioxidant, antiradical and antimicrobial activities suggest the use of individual fractions from aqueous extracts of sweet chestnut, olea and cynara for phytoterapics and cosmetics, both in order to prolong the shelf life of products in combination or in place of the traditional chemicals, and as active ingredients. Phytoterapics, cosmetics and nutraceuticals Accademia dei Georgofili, Firenze – 13 novembre 2015

  13. Accademia dei Georgofili, Firenze – 13 novembre 2015

  14. OLIVE PIT EXTRACTION PROCESS Vegetable biomass and waste from olive-oil process CIRCULAR ECONOMY VEGETAL DISCARD TISSUES COMBUSTION GREEN ENERGY SOLID OLIVE WASTE VEGETAL WATER NOVEL FOODS LEAVES NATURAL ACTIVE COMPOUNDS PURE WATER WASTE WATER FUNCTIONAL FOODS REUSE

  15. Diafiltration Diafiltrated water Leaves/branches Washing waters Leaves/branches process Hot water extraction MF UF NF OI Pulps/husksprocess Extracted vegetal material Pre-filtration 60 L Waste Dosing Tanks Chopped olives, wet/dry pomaces diluted with waste water Ultrapure water Solid and semi-solid waste intended for the storage tank for subsequent use eg. fertilizers and biogas Marketed products 2 thermostated reactors Pre-treatment section PRODUCTS REFINING with RESINS Treatment plant for leaves/branches/pulp and dry/wet husks diluted with waste water

  16. Oleafractions: quali-quantitativecharacterization HPLC/DAD quantitative analyses of Olea plant fractions, concentrate fractions and powders (spray dried). Data are mean values of triplicate analyses (±SD). OH T der: Hydroxytyrosol derivatives; SI der: Secoiridoids derivatives; EA der: Elenolic Acid derivatives; HC der: Hydroxycynnamic derivatives; Verb: Verbascoside. Accademia dei Georgofili, Firenze – 13 novembre 2015

  17. Cynara ActiveR , nutraceutical standardized extracts from Cynara scolymus L. Scardigli A., Vignolini P., Pinelli P., Romani A. XXVI International Conference on Polyphenols, Polyphenols Communication 2012, Vol. I, pp. 267-268.

  18. Cynarafractions: quali-quantitativecharacterization HPLC/DAD quantitative analyses of different plant fractions from Cynara leaves (CUF and CRO), and two concentrated fractions from CRO: soft extract and spray dried from green leaves. Data are mean values of triplicate analyses (±SD). CUF = Concentrate of Ultrafiltration; CRO = Concentrate of Reverse Osmosis; GL = Green Leaves. Accademia dei Georgofili, Firenze – 13 novembre 2015

  19. Olea and Cynarafractions: antiradicalactivitywith DPPH assay Accademia dei Georgofili, Firenze – 13 novembre 2015

  20. Grapeseedsextract: quali-quantitativecharacterization Accademia dei Georgofili, Firenze – 13 novembre 2015

  21.  in vitro Antiradical Activity Antiradical Activity vs DPPH (50,7 μM) at 5 concentrations: 3,3- 8,3- 16,7- 25,1- 33,3 μM Gallic acid (EC50 7.75 M) Tannic acid (EC50 2.5 M) Grape seeds (EC50 5.75 M) Pine bark extract (EC50 5.25 M) Green tea extract (EC50 5.75 M) Myrtle aqueous extract (EC50 4.5 M) Myrtle hydroalcoholic extact (EC50 6.75 M) Catechin (EC50 12.5 M) Myricitrin (EC50 11.7 M)

  22. EGCG Tannins content and single compounds molecular weights in grape seeds extract Accademia dei Georgofili, Firenze – 13 novembre 2015

  23. Liquid and gel ormulationsforbiostatic/biocide and repellingproducts • Tests on Olea crops. • Repellingactivity • Repelling, biostatic/biocide activity, plantbiostimulantactivity: fieldtests on tobacconematodes • Antimicrobialactivitytowardselectedpathogenicbacterialstrains • Tests on soil Accademia dei Georgofili, Firenze – 13 novembre 2015

  24. FOOD ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES Use of tannins mixed with extracts from OleaEuropaea and Cynara replacing chemical additives

  25. Natural and qualityfoods Use of tannins mixed with extracts from OleaEuropaea and Cynara replacing chemical additives

  26. Waste water Energy ZERO waste Anaerobic fermetnation Organic fertilizer Polyphenols Vegetal water

  27. A circular economy seeks to rebuild capital, whether this is financial, manufactured, human, social or natural. This ensures enhanced flows of goods and services.

  28. Thank you Annalisa Romani Margherita Campo Elisa Fierini Francesca Ieri Stefano Mulas Patrizia Pinelli Arianna Scardigli Pamela Vignolini Chiara Vita

  29. LIFE13 ENV IT 000461 Polifenoli estratti dai sottoprodotti e reflui agricoli come biostimolanti e prodotti per la difesa: un’importante opportunità per ridurre i rifiuti e stimolare l’economia circolare Prof.ssa Annalisa Romani – Phytolab-DiSIA, Università di Firenze DIFESA INNOVATIVA DELLE PIANTE DAI LORO PATOGENI, NEL RISPETTO DELL’AMBIENTE E NELL’AMBITO DELL’ECONOMIA CICLICA Accademia dei Georgofili, Firenze – 13 novembre 2015

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