Biochemistry Lab. Protein Purity Assessment and Identification. Coomassie Staining. How does Coomassie interact with proteins?. Coomassie Staining. Benefits. Limitations. Western Blotting. Transfer – Block Apply Primary Antibody Apply Secondary Antibody. Western Blots.
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Protein Purity Assessment and Identification
How does Coomassie interact with proteins?
Transfer – Block
Apply Primary Antibody
Apply Secondary Antibody
Matrix Assisted Laser Desorption Ionization (MALDI)
Electrospray Ionization (ESI)
α-cyano-4-hydroxycinnamic acid (CCA)