Impact of hipO and dcuA Mutations on Transformation Frequency in Campylobacter jejuni

DESCRIPTION

This study investigates the natural transformation frequency in Campylobacter jejuni strains JB202 (mutated hipO) and BQ304 (mutated dcuA) compared to the wild-type 11168. Donor DNA carrying tetracycline (tet(O)) and kanamycin (aphA3) resistance markers were utilized in the experiments. The results indicate that mutations in hipO and dcuA do not significantly affect the natural transformation frequency in C. jejuni, suggesting that these genes may not play a major role in the transformation process.

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Feb 13, 2026

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Impact of hipO and dcuA Mutations on Transformation Frequency in Campylobacter jejuni

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A B Figure S1. Natural transformation frequency of JB202 and BQ304. Donor DNA with a tetracycline resistance marker [tet(O)] and kanamycin resistance marker (aphA3) were used in experiments A and B, respectively. The results showed that in comparison with wild-type 11168, mutation of hipO (JB202) and dcuA (BQ304) did not have a significant effect on the natural transformation frequency in C. jejuni.

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