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Supplementary Figure 1. G073. HUVECs. 40. 30. % PI +. 20. CD133 +. 10. E030. 0. control. TMZ 10 μ M. TMZ 50 μ M. TMZ 100 μ M. CD133+. Isotype control CD31-PE CD105-PE. G077. G408. 100. 20. 80. 15. 60. % PI +. % PI +. 10. 40. 5. 20. 0. 0. control. control.

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slide1

Supplementary Figure 1

G073

HUVECs

40

30

% PI +

20

CD133 +

10

E030

0

control

TMZ 10μM

TMZ 50μM

TMZ 100μM

CD133+

Isotype control

CD31-PE

CD105-PE

G077

G408

100

20

80

15

60

% PI +

% PI +

10

40

5

20

0

0

control

control

TMZ 10μM

TMZ 50μM

TMZ 100μM

TMZ 10μM

TMZ 50μM

TMZ 100μM

CD133-

HUVECs

E030

a

CD133-

Ki67

b

Supplementary Figure 2

a

b

PE

slide2

Supplementary Figure 3

2000

1500

/5000 beads

number of GBMs

1000

500

0

control 0Gy

control 5Gy

co-culture 5Gy

co-culture 0Gy

G008

G081

5000

4000

number of GBMs

3000

/5000 beads

2000

1000

0

control 5Gy

control 0Gy

co-culture 0Gy

co-culture 5Gy

G081

G008

G077

G008

G077

G081

60000

25000

60000

20000

40000

Co-culture 0Gy

Control 0Gy

Co-culture 5Gy

Control 5Gy

40000

15000

CFSE MFI

CFSE MFI

CFSE MFI

10000

20000

20000

5000

0

0

0

control 5Gy

control 0Gy

control 5Gy

control 0Gy

control 0Gy

control 5Gy

co-culture 5Gy

co-culture 5Gy

co-culture 0Gy

co-culture 0Gy

co-culture 5Gy

co-culture 0Gy

a

G077

1750

1500

1250

number of GBMs

/5000 beads

1000

750

500

250

0

control 5Gy

control 0Gy

co-culture 5Gy

co-culture 0Gy

b

CD133 - APC

Isotype control - APC

co-culture

CD133-APC

control

CFSE-FITC

c

CFSE

slide3

Supplementary Figure 3

G077

G008

G081

35

55

15

50

30

45

40

G077

25

10

35

20

30

% specific cell death

% specific cell death

3500

% specific cell death

25

15

20

5

3000

10

15

10

5

2500

5

0

0

0

2000

/5000 beads

number of GBMs

1500

control 5Gy

control 5Gy

control 5Gy

co-culture 5Gy

co-culture 5Gy

co-culture 5Gy

1000

500

0

control

co-culture

control 5Gy

co-culture 5Gy

control 100μM TMZ

co-culture 100μM TMZ

control 100μM TMZ 5Gy

co-culture 100μM TMZ 5Gy

d

e

slide4

Supplementary Figure 4

0Gy

15Gy

5Gy

HUVECs

E030

100

3

G1

S

80

G2

2

60

% PI+

% of cells

40

1

20

0

0

0Gy

5Gy

15 Gy

control

50μMTMZ

100μMTMZ

E030

5Gy

0Gy

15Gy

7%

4%

1,5%

PI

5Gy

15Gy

0Gy

HUVECs

25

day 1

day 4

20

day 7

15

HUVECs

% Pi +

10

5

5Gy

15Gy

0Gy

0

0Gy

5Gy

15Gy

E023

E030

100

G1

S

80

G2

60

% of cells

40

20

0

50μMTMZ

100μMTMZ

control

a

b

c

d

SSC

FSC

e

slide5

G081

G008

G408

f

Supplementary Figure 5