April 16, 2012. BellRinger Objective Explore the use of restriction enzymes in genetic engineering Describe the set-up and use of electrophoresis Homework Bring in a 9V battery Complete pre-lab. Biotechnology. A Brave New World.
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human genome3.2 billion bases
Our tool kit…
How have theselittle guys gotten to be so diverse??
mix heat-killed pathogenic & non-pathogenic
gene we want
cut plasmid DNA
insert “gene we want” into plasmid...“glue” together
Madam I’m Adam
Able was I ere I saw Elba
a man, a plan, a canal, Panama
Was it a bar or a bat I saw?
go hang a salami I’m a lasagna hog
Madam I’m AdamRestriction enzymes
“new” protein from organism
ex: human insulin from bacteria
How can bacteria read human DNA?Why mix genes together?
human insulin gene in bacteria
DNA RNA protein trait
Jelly fish “GFP”
But it would be so much easier if we didn’t have to use bacteria every time…
No more bacteria,No more plasmids,
No more E. colismelly looks!
What does 90°Cdo to ourDNA polymerase?
play DNAi movie