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ANA 812. Digestive System III: Liver, Gallbladder & Pancreas. Bethany Cox Sarah Marks Joe Slusher Kara Stebbins. Slide #106 Liver- Pig 4x. Portal Area. Liver Lobule. Slide #106 Liver- Pig. 10x. Central Vein. 40x. Central Vein. Cords of Hepatocytes Liver Sinusoids
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ANA 812 Digestive System III: Liver, Gallbladder & Pancreas Bethany Cox Sarah Marks Joe Slusher Kara Stebbins
Slide #106 Liver- Pig 4x Portal Area Liver Lobule
Slide #106 Liver- Pig 10x Central Vein 40x • Central Vein • Cords of Hepatocytes • Liver Sinusoids • Binucleate Cell
Slide #106 Liver- Pig 4x 40x • Portal Vein • Hepatic Artery • Lymphatic Vessel • Bile Ductule
Slide #106 Liver- Pig-40X • Portal Vein • Lymphatic • Bile Ductule • Hepatic Artery
Slide #101 Liver- Human-4X • Note relative • lack of connective • tissue.
Slide #101 Liver- Human-10X • Central Vein • Liver Sinusoids • Hepatocyte Cords • Lipid Droplets
Slide #104 Liver- Mouse 40x 4x Portal Vein Bile Ductule Lymphatic Vessel
H&E stain (human, slide 101) Trypan blue stain with neutral red (monkey, slide 103) Liver: Von Kupffer cells-40X Large oval nuclei projecting into sinusoids Trypan blue phagocytized into granules
Normal liver (H&E stain, human, slide 101) Cirrhosis (Masson’s trichome stain, human, slide 107) Liver: One Effect of Cirrhosis-40X Vacuolated hepatocyte cytoplasm Bile canaliculi
Normal liver (H&E stain, human, slide 101) Cirrhosis (Masson’s trichome stain, human, slide 107) Liver: Another Effect of Cirrhosis-4X Blue-staining collagen (fewer hepatocytes, more CT)
Slide #178 – Pancreas and Suprarenal Gland – Monkey (Azan Stain) – 4X • Pancreatic Islets of Langerhans (endocrine portion of the pancreas) • Ducts • Intercalated • Intralobular • Interlobular
Slide #178 – Pancreas and Suprarenal Gland – Monkey (Azan Stain) – 40X • Islet of Langerhans 4X
Slide #178 – Pancreas and Suprarenal Gland – Monkey (Azan Stain) – 10X • More secretory acinar divided into lobules • Ducts • Intercalated (not seen at this power) • Intralobular • Interlobular
Slide #104-Gallbladder and liver-10X • Simple columnar eplithelium • Lamina propria • Smooth muscle layer • Definitive mucosal folds Monkey, H&E stain
Slide #105-Gallbladder -10X • Human specimen after death • Extremely folded gallbladder • mucosa seen here is typical of • human gallbadder Human, H&E stain
Comparison of Slides #104/#105-10X Monkey, H&E stain Human, H&E stain