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Gregor 2007. Use GFP- Bcd to further 2005 work Use time-lapse two-photon microscopy Fast and sensitive See Bcd on nuclei and anterior to posterior gradient. Gregor 2007. Fluorescence increases with time . Quick Summary.

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gregor 2007
Gregor 2007
  • Use GFP-Bcd to further 2005 work
  • Use time-lapse two-photon microscopy
    • Fast and sensitive
  • See Bcd on nuclei and anterior to posterior gradient
gregor 20071
Gregor 2007
  • Fluorescence increases with time
quick summary
Quick Summary
  • Bcd pattern seems to be established early and then stay regardless of
    • Nuclear proliferation
    • Nuclei size changes
    • Appearance of cell membranes
  • Rises and falls with mitosis
    • Stays constant within for different divisions
  • D is really small
  • Propose a new model that specifies nuclear degradation
castle
Castle
  • More recently have shown that characteristic lengths of source ~ gradient length
  • Suggest there might be localized production necessary to give rise to patterns
  • Big focus on methods  take issue with the photobleaching in Gregor 2007
  • Model their work and find their estimate of D is off and diffusion model still holds
is gfp bcd functional normal
Is GFP-Bcd functional/normal?
  • Yes.
  • GFP-Bcd can rescue a knockout
  • Pattern the same as wt
  • Stain for endogenous and GFP Bcd show colocalization
getting strange
Getting strange
  • Bcd associated with nuclei seems to spread out during mitosis?
  • Pretty constant
slide18

Is it just binding/unbinding from nuclei?

  • Use photobleaching to show recovery
    • Implies transport across nuclear membrane
    • And also degredation
  • Make a new model