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بسم الله الرحمن الرحيم

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بسم الله الرحمن الرحيم. GENUS: CORYNEBACTERIUM Prof. Khalifa Sifaw Ghenghesh. Diptheroides = Coryneforms Gram +ve, Nonmotile Rods. Arranged in V forms or Palisades Catalase +ve. 1. Corynebacterium diphtheriae. Diphtheria. Pleomorphic Bacilli Cells Contain Metachromatic Granules.

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slide1

بسم الله الرحمن الرحيم

GENUS: CORYNEBACTERIUM

Prof. Khalifa Sifaw Ghenghesh

slide2
Diptheroides = Coryneforms
  • Gram +ve,
  • Nonmotile Rods.
  • Arranged in V forms or Palisades
  • Catalase +ve
1 corynebacterium diphtheriae
1. Corynebacterium diphtheriae
  • Diphtheria.
  • Pleomorphic Bacilli
  • Cells Contain Metachromatic Granules.

Morphology

cultural characteristics
Cultural Characteristics
  • i. Loeffler's Serum Slope.
  • ii. Tellurite BA.

- Temp. 37oC,

- Aerobic and Facultative AnO2.

slide6
Corynebacterium diphtheriae forms black colonies on tellurit agar (left), on blood agar colonies appear white (right)
pathogenicity
PATHOGENICITY
  • Diphtheria is a Toxaemia.
    • Exotoxin: M. wt 62000
    • 2 Fragaments >> A & B
    • Inhibits Protein Synthesis.
    • Has Special Affinity for Certain Tissues "Mycocardium, Adrenals, Nerve Endings".
  • Toxin >>Toxoid.
  • Site of Infection >>Faucial.
    • Toxin >>Pseudomemebrane.
laboratory diagnosis
LABORATORY DIAGNOSIS
  • Important>>Specific Treatment Should Be Started Immediately on Suspicion of Diphtheria.
  • Specimen:Two Swabs from the Lesion.
  • Culture:

i. Loeffler's Serum Slope >> Albert Stain.

ii. Tellurite BA.

iii. BA>> Streptococcal or Staphylococcal Pharyngitis.

pathogenicity tests
PATHOGENICITY TESTS
  • In-vivo Tests:

i. Subcutaneous Test:

ii. Intracutaneuos Test:

  • In-vitro Test:
    • Elek Test ( Gel Precipitaion Test).
  • Tissue Culture:
treatment
TREATMENT
  • i. Antitoxin:> Moderate Cases:

> Severe Cases:

  • ii. Antibiotic:
  • i. Active Immunization >> PDT.
  • ii. Passive Immunization:
  • iii. Combined:

PREVENTION

schick test
SCHICK TEST
  • > Injection of 0.2 ml of Diphtheria Toxin Intradermally.
  • > Positive Reaction >> Erythema + Swelling
schick test1
SCHICK TEST

______________________________________________________________________

Result Test Arm Control Arm Interpretation Diphthri (Toxin) Inactivated Toxin Immunization

36h 120h 36h 120h

______________________________________________________________________

Negative -- -- -- -- Immune, Not

Not Hypersensitive Required

----------------------------------------------------------------------------------------

Positive + + -- -- Not Immune, Not Required

Hypersensitive

---------------------------------------------------------------------------------------------------------------------

Negative + -- + -- Immune Not

Hypersensitive Required

---------------------------------------------------------------------------------------------------------------------

Positive& + + + -- Not ImmuneContra-

PseudoHypersensitive indicated

______________________________________________________________________

slide14
Listerosis.
  • G+ve Coccobacilli >in Short Chains.
  • Motile at 25oC But Not at 37oC.
  • Aerobic to Microaerophilic.
  • On BA>Narrow Zone of b-Haemolysis.
  • Listerolysin O >
disease
DISEASE
  • Incidence and Transmission >
  • i. In Adults:
  • ii. Neonatal Infections:

a. Early Type:

b. Late Type:

laboratory diagnosis1
LABORATORY DIAGNOSIS
  • i. Specimen:Depend on Syndrome: Blood, CSF, Genital Tract Secretions.
  • ii. Media:Selective Media.
  • Penicillin, Ampicillin.

TREATMENT

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