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Circulating Cell-Free DNA

Circulating Cell-Free DNA. Amos Douvdevani, PhD Department of Clinical Biochemistry and Pharmacology, Soroka Medical Center & Ben-Gurion University of the Negev.

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Circulating Cell-Free DNA

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  1. Circulating Cell-Free DNA Amos Douvdevani, PhD Department of Clinical Biochemistry and Pharmacology, Soroka Medical Center & Ben-Gurion University of the Negev • Necrosis, apoptosis, and possibly “active secretion” are thought to be the mechanisms by which cell-free nucleic acids enter the circulation. 0.18Kb

  2. Direct fluorometric Method for DNA Measurement Goldshtein et al. Ann Clin Biochem 2009; 46: 488–494 SYBR Gold stain Serum/ plasma 20 l Measurement of fluorescence Dilution of sera in buffer containing SYBR Gold stain • Mix & Measure, immediate quantitative results • Cost of reagents $5/100 samples • Circulating free DNA Could be tested in the clinic using a small device (DNAmeter)

  3. Few ideas for Possible collaboration • Diagnosis of pneumonia (reduce X-ray) • Diagnosis of flue and other viral diseases • Early cancer detection • Any idea you have (free assay for all) Amos Douvdevani, amosd@bgu.ac.il, Tel: 054-4403214

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